Tyrosine Sulfation Specific Antibody Discovery Service

Do you face difficulties with identifying and characterizing proteins that undergo tyrosine sulfation? Creative Biolabs' Tyrosine Sulfation Specific Antibody Discovery Services deliver high-affinity specific antibodies to expedite your research work. Our advanced solutions produce essential research tools that help you fast-track your studies.

Background

As a post-translational modification (PTM) proteins receive a sulfate group that attaches to their tyrosine residues. The essential process of protein-protein interactions in biological systems depends on the catalytic activity of tyrosylprotein sulfotransferases (TPSTs). The attachment of a sulfate group to tyrosine residues causes substantial changes in their biochemical properties, which affect their participation in electrostatic interactions and hydrogen bonding. Protein functional integrity and molecular interactions with receptors and ligands depend on modifications to its three-dimensional formation. According to Hoffhines et al. (2006), many tyrosine-sulfated proteins serve important functions in cell signaling, blood coagulation, and immune responses, though researchers have yet to identify a substantial number of these proteins. The primary reason many of these modified proteins have remained unknown is the historical absence of effective research tools for their discovery and isolation. Such tools need to be developed because the process of tyrosine sulfation does not follow a template-driven sequence, which prevents direct prediction from genetic sequences. We meet this essential requirement by delivering high-quality antibodies that accurately bind to tyrosine-sulfated proteins, allowing researchers to investigate this significant modification.

Fig.1 The tyrosylprotein sulfotransferase reaction.^{2, 4}

Fig. 2 Protein tyrosine sulfation (PTS) and its biological path. ^{3, 4}

Antibody Types

Creative Biolabs delivers a range of monoclonal and polyclonal antibodies generated using rabbit, mouse, or sheep host animals. Our antibody offerings include:

• Site-Specific Anti-Tyrosine Sulfation Antibodies: These antibodies target a specific tyrosine sulfation region within a protein by binding to it.
• Pan Anti-Tyrosine Sulfation Antibodies: The pan anti-tyrosine sulfation antibodies identify tyrosine sulfation sites without regard to adjacent amino acid sequences.

Discovery Strategy

Creative Biolabs uses three primary methods to develop Tyrosine sulfation-specific antibodies.

• Monospecific Anti-Tyrosine Sulfation Polyclonal Antibody Production: In this classical technique, researchers immunize animals to produce an antibody response. Our optimized immunogen design and protocol enable us to achieve maximal specificity and sensitivity in polyclonal antibody production.

• Phage Display Strategy for Anti-Tyrosine Sulfation Monoclonal Antibody Discovery: Phage display technology enables researchers to generate extensive antibody fragment libraries and conduct screenings to pinpoint fragments that bind specifically to tyrosine-sulfated proteins. This technique excels at producing antibodies that exhibit both high affinity and precise binding specificity.

• Hybridoma Strategy for Anti-Tyrosine Sulfation Monoclonal Antibody Discovery: The hybridoma technique produces monoclonal antibodies by merging B cells that produce antibodies from vaccinated animals with myeloma cells to generate immortal cell lines. Our advanced hybridoma technologies enable us to produce stable and high-quality monoclonal antibodies.

Types of PTM

We provide tyrosine sulfation specific antibodies and antibodies targeting a variety of PTMs, as shown in the table below.

Why Choose Us?

Creative Biolabs stands as the perfect collaborator for discovering antibodies that target tyrosine sulfation. We offer:

• Expertise: Our team possesses advanced expertise and experience in the development of antibodies with a focus on post-translational modifications such as tyrosine sulfation.
• Customization: Our services adapt specifically to your research requirements to deliver the most fitting antibody for your application.
• Advanced Technology: Through our application of innovative phage display and hybridoma generation technologies, we are able to produce antibodies that exhibit both high affinity and specificity.
• High-Quality Products: Our company ensures the delivery of antibodies that consistently achieve the top levels of quality and performance standards.
• Published Data: Published data in peer-reviewed publications demonstrate that our services have been instrumental in numerous successful research projects.

FAQs

Q: Are the antibodies suitable for my particular assay needs, such as Western blotting and immunoprecipitation?

A: The antibodies we offer are adaptable for numerous different research applications. Our team offers comprehensive information along with expert recommendations to match your specific assay needs. Contact our team for help.

Q: How do your antibody products stack up against the options that you can purchase commercially?

A: Creative Biolabs produces specific high-affinity antibodies designed to detect challenging targets such as post-translational modifications. We customize antibodies according to your specific requirements, which sets us apart from typical pre-made products. Let us know your requirements.

Q: How should I prepare my protein samples for analysis?

A: Sample preparation varies by application. Our experts offer guidance and assistance that help achieve the best possible results. Contact us for specifics.

Q: How long does it usually take for your custom antibodies to be produced?

A: Antibody generation duration varies based on the complexity of the target and the production approach. Our team delivers high-quality antibodies through efficient work processes to ensure rapid delivery. Reach out to us so we can evaluate your project and provide an estimate.

Q: What steps should I take if the antibody fails to perform as anticipated during my experiment?

A: Creative Biolabs commits to customer satisfaction through complete technical support designed to resolve any problems. Contact us, and we'll find a solution.

Creative Biolabs delivers complete Tyrosine Sulfation-Specific Antibody Discovery Services along with numerous solutions to create research-specific high-quality antibodies. Our commitment stands with assisting you in speeding up your research process and attaining your scientific objectives. Contact us now for more details.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.