Synthetic Attenuated Virus Engineering (SAVE)

The advances in synthetic biology pave the way for the creation of new vaccine candidates for various potential viruses. Using synthetic attenuated virus engineering (SAVE), the recoding of viral genomes so that codon pairings, which are normally under-represented in the genome space of a particular organism, become over-represented. As these unfavorable codon pairings accumulate within the genome, the virus becomes attenuated.

Creative Biolabs presents a breakthrough approach to live-attenuated vaccine design. We use our breakthrough platform technology termed Synthetic Attenuated Virus Engineering (SAVE) to construct live-attenuated viral vaccines against multiple targets. The SAVE platform relies on synthetic biology and the rational re-design of a target virus entire genome to yield a vaccine strain.

Advantages of SAVE Strategy in Developing Live Attenuated Virus Vaccine Candidates

Several approaches to vaccine design exist, including inactivated and live attenuated vaccines. Live attenuated vaccines promote long-term immunity by activating cellular and antibody responses. Using the technology developed in synthetic biology, attenuated poliovirus and influenza virus have been synthesized and shown protection against in mice from infection. The live attenuation of these viruses is achieved by a novel approach to vaccine design, synthetic attenuated virus engineering (SAVE), in which changes to the preferential usage of certain codons and codon pairs to encode amino acids and adjacent amino acid pairs have been incorporated into the virus during synthesis. By selecting the degree of under-represented codon or codon pair incorporated, which also results in the concomitant increase in rare CpG and UpA dinucleotide frequencies, viruses can be attenuated in both cell culture and mouse models in a tunable fashion. This method has been extended to HIV retrovirus and bacteria, Streptococcus pneumoniae. With the increased pace of advance in vaccine designs, a view that includes disease eradication rather than just control is advocated. With initial positive results in viral attenuation using SAVE, investigations in other virus families may show that SAVE have a broad applicability.

• Universality: Successfully applied to many different viral species;
• Effectiveness: The protection is equal or better;
• Rapid and timely vaccine preparation: Computer-aided rapid, systematic design and mass production of vaccine viruses in tissue culture;
• Adjustability: The recoding can be customized to allow varying degrees of attenuation; different gene segments can be re-encoded in different ways and combined in one candidate vaccine;
• Gene stability: The probability of mutation reversion is very low.

Our Synthetic Attenuated Virus Engineering (SAVE) Platform

• Rational, large-scale recoding of viral genome using a computer algorithm;
• DNA chemical synthesis;
• Cloned into viral cDNA;
• In vitro RNA transcription;
• Transfection into cells.

Creative Biolabs is a world leader in the field of vaccine and provides different kinds of live-attenuated vaccine design services to meet our customer's demand. Using our breakthrough SAVE platform technology, you will construct a higher performance live-attenuated viral vaccine. Our scientists are confident in offering the best services upon request!

All of our products can only be used for research purposes. These vaccine ingredients CANNOT be used directly on humans or animals.