Custom Bacteriophage Conjugation Service

As a leading global provider of bioconjugation services, Creative Biolabs focuses on providing custom bacteriophage conjugation services to customers around the world. Relying on our state-of-the-art manufacturing facilities and decades of conjugation experience, we are confident to meet your needs for creating novel conjugates that can be quickly and cost-effectively added to your value chain.

Fluorescent Labeling of Bacteriophage

Bacteriophage and viruses are composed of protein-encapsulated DNA or RNA, so they are suitable for fluorescent labeling. Fluorescent proteins are playing a vital role in cell biology research. Fluorescent protein-labeled bacteriophages are closely linked to molecular imaging of bacteriophages. Labeled bacteriophage can be used to identify bacteriophage fate in vivo, such as bacteriophage translocation and retention in cells, tissues and organs. Bacteriophages labeled with the green fluorescent protein (GFP) have been reported to emit light upon excitation and can be detected in mouse organs. Furthermore, increasing evidence suggests that red fluorescent protein (RFP)-labeled bacteriophage provides an attractive tool for studying bacteriophage-cell and tissue interactions. Additionally, fluorescently labeled bacteriophages are very efficient methods of detecting and quantifying specific bacterial groups in mixed microbial communities.

Fig.1 Scheme of RFP labeling of bacteriophages by bacteriophage display method.

Bacteriophage-Drug Conjugation

Bacterial resistance to traditional antibiotics is a public health problem, but the lack of selectivity limits the use of extremely potent antimicrobials. The new application of bacteriophage as a targeted drug carrier provides a new idea to solve this problem. For example, a novel drug conjugation strategy was developed based on the attachment of hydrophobic drugs to bacteriophages via antibiotics that act as branched linkers for solubility enhancement. This new formulation enables the drug-loading capacity of bacteriophages to be significantly improved, thereby improving their performance as targeted drug-loading nanoparticles. Importantly, bacteriophages are the workhorse of antibody engineering and have attracted a lot of attention in nanobiotechnology.

Custom Bacteriophage Conjugation Services at Creative Biolabs

In addition to the conventional conjugation of fluorescent proteins and drug molecules, we also focus on the design of fluorescent bacteriophage sensors. Fluorescence-based sensors are an attractive class of molecular devices that can be used to analyze a variety of samples. Considering the relatively small size of bacteriophages, their binding to nanoparticles or quantum dots may not yield effective results. Therefore, Creative Biolabs specializes in the design services of a variety of bacteriophage sensors. Our professional team explores some interesting fluorophores that can efficiently construct their bioconjugates with bacteriophages.

Process of Our Conjugation Services

Creative Biolabs has sufficient knowledge of the reactive groups, reagent types and fluorescent properties of bacteriophage conjugation. We are committed to providing one-stop bioconjugation services, from biomolecule preparation to final conjugate purification. At Creative Biolabs, custom bioconjugate reagents are produced under strict quality control processes to ensure complete customer satisfaction. We will provide reasonable and applicable solutions and coupling methods according to your project needs. Please contact us in time for more information.

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