Custom Oligonucleotides-Peptide Conjugation Service

As an expert in the field of peptide-oligonucleotide conjugates (POCs), Creative Biolabs has experienced experts and leading technologies. We have been committed to providing cost-effective POCs services. We strictly control the quality of products during the production process, and the final products are analyzed through different technologies to ensure the best products are delivered to customers.

Overview of POC

POCs are covalent constructs that link an oligonucleotide or its analog to a synthetic peptide sequence. Despite the high costs, POCs represent an increasingly successful strategy. POCs consistently exhibit strong biological effects in various applications. They involve modified peptides or proteins. POCs play a crucial role in the studies of the protein- and peptide-nucleic acid interaction. In addition, they can be used to improve cell-specific and intracellular delivery of antisense or antigene compounds. Notably, this technology is playing an increasingly important role in cancer immunotherapy, liquid biopsy, and single-cell analysis. Briefly, POCs can be used:

Increase the stability of the oligonucleotide
Template-enhanced POC coupling to larger molecules
As aptamers and nanomaterials
Intracellular delivery

Synthetic Strategies for POCs

There are two main approaches for synthesizing POCs, which are conceptually divided into on-resin synthesis of the entire hybrid molecule, and post-synthetic conjugation either in solution or on the solid phase, especially through click chemistries. The first method relies on the sequential coupling of amino acid monomers and nucleic acid monomers to generate diblock copolymers. The second method is more commonly used because it circumvents compatibility issues with solid-phase synthesis protocols and does not rely on foreign protecting group strategies. In this approach, each molecule is synthesized individually with unique reactive functional groups. The two moieties are then ligated directly followed by subsequent purification of the desired POC.

Fig. 1 Chemical approaches for POC synthesis.

Our Services

In fact, the conjugation of these macromolecules and functionalized molecules is very difficult. At Creative Biolabs, we are confident to offer different strategies for synthesizing POCs, including solid-phase synthesis, liquid-phase synthesis, and biosynthesis. Moreover, our chemoselective ligation method facilitates efficient ligation and reaction between oligonucleotides and peptides. The resulting POCs were purified by high-performance liquid chromatography (HPLC) and characterized by mass spectrometry. In addition, we also provide a variety of customized services, including but not limited to:

Individual synthesis and purification of oligonucleotides and peptides
Chemical conjugation of individual components via reactive linkers
Characterization and validation of POCs

With advanced equipment and a professional team, Creative Biolabs has been committed to providing one-stop POCs services. Our dedicated scientists will provide customized POCs based on the specific needs of your project to keep your project running smoothly. If you are interested in our POCs service, please feel free to contact us for more details.