FAQ

Bioconjugation is the process of combining two or more molecules to create a new composite with the characteristics of a single component. Bioconjugation techniques have impacted nearly every discipline within the life sciences. The application of novel conjugates with specialized activities through conjugation reactions and crosslinking reagent systems has enabled the assay of minute quantities of substances, the in vivo molecular targeting, and the modulation of specific biological processes.

Some common bioconjugation reactions include:

• Amine Reactions: These reactions involve the coupling of a lysine or N-terminal amine group on a biomolecule to a molecule containing a reactive group such as an NHS-ester or isothiocyanate.
• Sulfhydryl Reactions: These reactions include the coupling of sulfhydryl groups (from cysteine) on biomolecules with molecules containing maleimide or pyridine disulfide groups.
• Aldehyde Reactions: These reactions involve the coupling of glutamic acid, aspartic acid, or C-terminal aldehyde group on biomolecules with molecules containing hydrazide or oxime groups.
• Click Chemistry Reactions: These reactions, such as copper-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC), are commonly used for bioconjugation due to their high efficiency and selectivity.

With the development of novel cross-linking techniques and reagents, bioconjugation technology has important applications in many fields, including biochemical assays, drug delivery, in vivo imaging, industrial applications, and biological interactions. This provides important tools and methods for research, diagnosis, and therapy.

We can provide one-stop bioconjugation services, including but not limited to protein/antibody, nucleic acids/oligonucleotides, small molecule, bacterial, viral, and bacteriophage-related conjugations.

Choosing the right conjugation technique depends on several factors, including:

• The properties of the molecules you want to conjugate (e.g., their functional groups, solubility, and stability)
• The stability, specificity, and efficiency of the conjugate
• The intended application.

It may also be helpful to consult with our experts or refer to the literature for guidance on selecting the right conjugation method for your specific needs.

A bioconjugation project can typically take around 4-10 weeks to complete, but this timeline can vary based on the specific requirements of the project, including the complexity of the project, the specific conjugation methods being used, the number of molecules being conjugated, the scale of the project, etc.

We strictly control the quality of source materials. Additionally, Our well-established protocols govern every aspect of the preparation, purification, and characterization processes, enabling us to deliver consistent, reliable products.

Yes, our expert team can provide a tailored bioconjugation service to match your specific demands. These include but are not limited to protein, nucleic acid, and small molecule-related custom conjugation services.

You need to provide the following information when placing an order, including:

• The project description or product code(s)
• Product quantity
• Shipping and billing address
• Purchase Order Number or credit card
• Telephone number, email address, and/or fax number

During transit, bioconjugates are typically shipped at ambient temperature or on dry ice to ensure their stability. Upon receipt, it is essential to adhere to the recommended storage conditions, which can range from room temperature to -20°C or below, depending on the specific product. To prevent degradation and maintain optimal activity, it is advisable to store the bioconjugates in aliquots, avoiding repeated freeze-thaw cycles. Furthermore, photosensitive products necessitate storage away from light exposure. For detailed storage requirements, please consult the product datasheet or the accompanying instructions.

We recommend first verifying whether the product is still within its usable lifespan and ensuring that the storage conditions have been properly maintained. If these factors appear to be in order, please contact us. Our team of experts is available to provide post-sale consultation and can analyze the specifics of your problem, helping you quickly identify the root cause and offering potential troubleshooting solutions to address the issue.

We offer comprehensive post-sale support and consultation services. Our dedicated team is available to provide solutions to questions or concerns you may have and to provide guidance on the optimal use of our products. Please contact us if you require any assistance.