Custom Protein-Oligonucleotide Conjugation Service

Protein-oligonucleotides conjugation presents great potential in diagnostic and treatment applications. With years of experience, our scientists at Creative Biolabs have developed a series of methods to meet the specific needs of each customer with the best project design.

Introduction of Antibody-Oligonucleotide Conjugates (AOCs)

Recombinant techniques allow the combination of different functionalities into one entity. What's more, the introduction of nonprotein labels can further extend the functionality. For example, the conjugation of fluorescent labels or cytotoxic agents facilitates the development of highly sensitive detection methods and targeted therapies.

Antibodies can specifically recognize and bind antigens to protect the body against invading pathogens. Oligonucleotides are short RNA and DNA molecules that contain two important parameters, including the number of nucleotide bases and their composition. Antibody-oligonucleotide conjugates (AOCs) refer to a novel type of synthetic chimeric biomolecules that have received more and more attention in multiple fields. By combining the tissue/disease selectivity of monoclonal antibodies (mAbs) with the precision of oligonucleotides, the novel developed AOCs are able to more effectively target underlying genetic drivers of disease.

Applications of Antibody-Oligonucleotide Conjugates (AOCs)

Till now, AOCs have played an important role in a variety of diagnostic and therapeutic applications, which including but not limited to:

Immuno-PCR (iPCR)
Proximity extension assay (PEA)
Electrochemical proximity assay (ECPA)
DNA-PAINT imaging
Protein arrays
Therapeutics

In diagnostic applications, PCR can amplify the number of DNA markers, which greatly improves the detection limit. In therapeutic applications, AOCs can serve as therapeutic agents containing ASO or siRNA as the payload and as a pretargeting module for radionuclide therapy and imaging.

Conjugation Strategies

Similar to antibody-drug conjugation (ADC), there are both covalent and noncovalent approaches for the preparation of AOCs. Noncovalent AOCs refer to modular constructs with mAb and ON molecules connected through a special linker. Bioconjugates based on non-covalent interactions have general applicability and ubiquity for analytical applications and screening. Corresponding, covalent approaches allow for more stable linkages and provide more defined structures. Moreover, a variety of cleavable linkers and non-cleavable linkers are all available. Creative Biolabs provides a variety of approaches which including but not limited to:

Fig. 1 Simplified schematic representation of AOC.

Noncovalent Approaches:
Streptavidin−Biotin System, Protein A and Protein G, Protamine System, Chemical Protamine Conjugation to mAb.
Covalent Approaches:
Conjugation on Lysine Residues of Antibodies, Amine-to-Thiol Coupling, Hydrazone Coupling, DNA-Templated Protein Conjugation (DTPC), Conjugation on Cysteine Residues of Antibodies. Inverse Electron-Demand Diels−Alder Reaction (iEDDA), Strain-Promoted Alkyne−Azide Coupling (SPAAC), Conjugation on Cysteine Residues of Antibodies.

Creative Biolabs offers the most advanced and comprehensive protein-oligonucleotides conjugation services today. We work to amplify your success in a highly collaborative manner. If you are interested in our services, please do not hesitate to contact us for detailed information.