Custom Protein-Oligosaccharide Conjugation Service

Protein-oligosaccharide conjugation is the most widely used method for antibody generation against oligosaccharides on glycoproteins. Our team of experts at Creative Biolabs can assist you in designing the best study outline customized to meet the requirements of your drug discovery/development program.

Protein-Oligosaccharide Conjugation Services

Oligosaccharides are saccharide polymers containing two to ten monosaccharides. They usually exist in the form of glycans and play important roles in cell recognition and cell adhesion. The low immunogenicity of carbohydrate antigens makes it difficult to elicit an effective immune response. This problem can be solved by covalently linking it to an immunological protein. Protein-oligosaccharide conjugates are also novel prebiotics to maintain gut health. These designed conjugates selectively target probiotics in the colon to enhance their competitive advantage in the microbiota.

Saccharide Conjugate Vaccines

Carbohydrates are widely distributed surface molecules that play important roles in various life processes, including cellular recognition and signaling. What's more, the role of carbohydrates in immune recognition enables broad application in vaccine development. Polysaccharides, especially β-glucan and dextran, can serve as vaccine carriers to deliver antigens into immune cells.

Galactosylated Modification

Galactose is an isomer of glucose and can be obtained from the hydrolysis of lactose under the catalysis of β-lactase. In oncology drug development, galactosylated stearate has been used to glycosylate vesicles for liver targeting.

Glucan Conjugation

Glucans refer to glucose polymers as dominant photosynthetic products. Glucan is a hydrophilic polysaccharide with medium to high molecular weight. Glucan is low immunogenic and biologically inert. Glucan can be conjugated to biotin or various fluorophores for a variety of functions, including neuronal tracing in living cells and cell lineage tracing in living cells.

Protein-Oligosaccharide Conjugates Method

Protein-oligosaccharide conjugation through the use of dicarboxylic acid linkers is the simplest and most efficient method. During this process, free amino groups are introduced to the oligosaccharide antigen to facilitate coupling to the bifunctional linker upon reaction with the disuccinimidyl ester. The obtained monosuccinimidyl ester of the oligosaccharide antigen then reacts with the free amino groups of the carrier protein to finally complete the coupling of oligosaccharides to proteins. What's more, the oligosaccharides with amine-containing linkers can be conjugated to carrier proteins according to two-step squaric acid chemistry. SELDI-TOF-MS or MALDI-TOF-MS can be used to monitor this conjugation reaction. Moreover, a variety of cleavable linkers and non-cleavable linkers are all available.

Fig. 1 Conjugation of protein-oligosaccharide.

Creative Biolabs offers the most advanced and comprehensive protein-oligosaccharide conjugation services available today. We work to amplify your success in a highly collaborative manner. If you are interested in our services, please do not hesitate to contact us for detailed information.