Custom Oligonucleotides-Bead & Particle Conjugation Service
Gold Nanoparticles-labeled ASOs
Antisense oligonucleotides (ASOs) have garnered significant attention as a promising tumor treatment, however, they necessitate a specialized carrier for efficient delivery into target cells. By binding gold nanoparticle-labeled ASOs to proteins, the positive charge and particle size are augmented, thereby enhancing the likelihood of cellular recognition and uptake. ASOs that undergo this coupling strategy exhibit superior stability and low toxicity, thereby enhancing drug delivery efficacy.
Fig.1 Representation of an AuNP for theranostics.
Magnetic Nanoparticles-labeled ASOs
By conjugating magnetic nanoparticles with specific biomolecules, they can serve as a powerful tool for separation and purification, such as the reversible conjugation of magnetic nanoparticles to DNA. Moreover, we provide you with the conjugate to separate or purify cells, nucleic acids, and proteins using magnetic nanoparticles.
Quantum Dots (QDs)-labeled ASOs
QDs have been progressively replacing organic fluorophores in various applications such as biosensing, imaging, and therapeutics, owing to their intrinsic material characteristics. Leveraging the distinctive properties of QDs and DNA, we are capable of delivering tailored QD-DNA conjugates to cater to your specific application requirements.
Fig.2 Conjugation of DNA to the shell of QD.
Silica Particles-labeled ASOs
Silica nanoparticles or silica microbeads are meticulously synthesized through a multi-step process to ensure their homogeneity and surface functionality. These synthesized silica beads exhibited a uniform spherical shape, with particle sizes spanning from 5 nm to 50 μm. By conjugating them with antibodies, proteins, or peptides, we can effectively cater to your diverse biomedical research requirements.
Applications
Bio-Bar Codes Assay (BCA)
BCA is a detection strategy that is known for its rapidity, high sensitivity, simplicity, and reproducibility. It operates by utilizing two types of probes that specifically target the desired analytes: magnetic microbeads (MMB) carrying biological probes and gold nanoparticles carrying molecules that bind to the target. Utilizing BCA as a diagnostic tool, we are pleased to provide you with the capability to detect proteins, nucleic acid targets, and small molecule compounds.
RNA Delivery
Nanoparticles designed to carry drugs offer the advantage of controlled release and precise targeting during drug delivery. Liposomes and micelles, which are lipid-based systems, represent the initial wave of drug-carrying nanoparticles and can incorporate gold or magnetic nanoparticles. These attributes further augment the delivery of RNA drugs, thereby enhancing therapeutic outcomes.
Leveraging our profound expertise in particle preparation and oligonucleotide (ASOs) conjugation, Creative Biolabs provides personalized services for ASO-bead & particle conjugation. Please trust in our professional team and advanced technology platform to seamlessly attach single- and double-stranded ASOs to any particles you desire. Should you encounter any challenges with your ASO-bead & particle conjugation project, please do not hesitate to contact us for expert guidance.
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