Custom Protein-Biotin Conjugation Service
Introduction of Biotin
Biotin is a water-soluble vitamin also known as vitamin B7, vitamin H or coenzyme R. Avidin is a glycoprotein found in egg white, which is known for its high affinity for biotin. However, due to its high isoelectric point and carbohydrate content, avidin can bind non-specifically to other substances besides biotin. Streptavidin is a protein with similar biological properties to avidin, derived from Streptomyces avidinii. It does not contain sugar chains, thereby reducing non-specific binding issues associated with avidin. The strength of the biotin-streptavidin interaction has made it a valuable tool for assay design and specific targeting studies.
Fig.1 Application of the biotin-streptavidin system in protein purification.
Application of Protein-Biotin Conjugates
The biotin-streptavidin system has the advantages of high specificity and strong binding capabilities, making it very versatile in a wide range of fields, including but not limited to:
Immunoassays
These include western blotting, immunoprecipitation, immunohistochemistry, etc. Biotin labeling significantly improves detection sensitivity compared to direct labeling with a detectable label.
Protein Purification
Utilizing a chromatographic column pre-fixed with streptavidin, biotin labeling can separate the target protein from the mixed solution.
Protein-Protein Interaction Assays
After immobilizing the proteins with the biotin-streptavidin system, the interaction between the two proteins can be analyzed by detection techniques such as surface plasmon resonance (SPR).
Our Protein-Biotin Conjugation Methods
- Biotinylated Crosslinkers
Creative Biolabs offers a diverse selection of biotin reagents tailored for labeling proteins and antibodies. Our range of moieties provides flexibility for various research and diagnostic applications.
| Product name | Carrying group | Reactive group(s) | Cat. |
| Biotin-LC-NHS Ester | NHS | NH2 | OLY-1023-YS192 |
| N-Succinimidyl D-Biotinate | NHS | NH2 | OLY-1023-YS193 |
| Biotin-PEG2-NHS Ester | NHS | NH2 | OLY-1023-YS213 |
| Biotin-PEG6-maleimide | Maleimide | SH | OLY-1023-YS195 |
| Biotin-PEG2-maleimide | Maleimide | SH | OLY-1023-YS209 |
| Biotin-PEG3-maleimide | Maleimide | SH | OLY-1023-YS210 |
| Biotin-C5-amine | NH2 | COOH, CHO | OLY-1023-YS199 |
| Biotin-PEG4-amine | NH2 | COOH, CHO | OLY-1023-YS204 |
| Biotin-PEG11-amine | NH2 | COOH, CHO | OLY-1023-YS207 |
| Biotin Hydrazide | Hydrazide | COOH, CHO | OLY-1023-YS184 |
| Biotin-LC-hydrazide | Hydrazide | COOH, CHO | OLY-1023-YS187 |
| Biotin-PEG4-hydrazide | Hydrazide | COOH, CHO | OLY-1023-YS205 |
| 6-Biotinamidohexanoic Acid | COOH | NH2 | OLY-1023-YS190 |
| Biotin-PEG4-propionic Acid | COOH | NH2 | OLY-1023-YS191 |
| Biotin-PEG3-acetic Acid | COOH | NH2 | OLY-1023-YS217 |
- BtAP-Tag® Site-Specific Biotinylation Platform
With our unique BtAP-Tag® in vivo biotinylation system, we can offer protein/antibody site-specific biotinylation services while eliminating heterologous antibody interference.
Creative Biolabs is a company that has long focused on the research and exploration of protein labeling technology. With a seasoned team of experts, we offer tailored protein-biotin conjugation solutions to meet the unique needs of your project. If you are interested in our services, please do not hesitate to contact us for a detailed description of our services and procedures. We look forward to collaborating with you!
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