Bioconjugation

Custom Protein-Biotin Conjugation Service

Introduction of Biotin

Biotin is a water-soluble vitamin also known as vitamin B7, vitamin H or coenzyme R. Avidin is a glycoprotein found in egg white, which is known for its high affinity for biotin. However, due to its high isoelectric point and carbohydrate content, avidin can bind non-specifically to other substances besides biotin. Streptavidin is a protein with similar biological properties to avidin, derived from Streptomyces avidinii. It does not contain sugar chains, thereby reducing non-specific binding issues associated with avidin. The strength of the biotin-streptavidin interaction has made it a valuable tool for assay design and specific targeting studies.

Fig.1 Schematic diagram of biotin for protein purification and analysis. (Creative Biolabs Original)Fig.1 Application of the biotin-streptavidin system in protein purification.

Application of Protein-Biotin Conjugates

The biotin-streptavidin system has the advantages of high specificity and strong binding capabilities, making it very versatile in a wide range of fields, including but not limited to:

Immunoassays

These include western blotting, immunoprecipitation, immunohistochemistry, etc. Biotin labeling significantly improves detection sensitivity compared to direct labeling with a detectable label.

Protein Purification

Utilizing a chromatographic column pre-fixed with streptavidin, biotin labeling can separate the target protein from the mixed solution.

Protein-Protein Interaction Assays

After immobilizing the proteins with the biotin-streptavidin system, the interaction between the two proteins can be analyzed by detection techniques such as surface plasmon resonance (SPR).

Our Protein-Biotin Conjugation Methods

  • Biotinylated Crosslinkers

Creative Biolabs offers a diverse selection of biotin reagents tailored for labeling proteins and antibodies. Our range of moieties provides flexibility for various research and diagnostic applications.

Product name Carrying group Reactive group(s) Cat.
Biotin-LC-NHS Ester NHS NH2 OLY-1023-YS192
N-Succinimidyl D-Biotinate NHS NH2 OLY-1023-YS193
Biotin-PEG2-NHS Ester NHS NH2 OLY-1023-YS213
Biotin-PEG6-maleimide Maleimide SH OLY-1023-YS195
Biotin-PEG2-maleimide Maleimide SH OLY-1023-YS209
Biotin-PEG3-maleimide Maleimide SH OLY-1023-YS210
Biotin-C5-amine NH2 COOH, CHO OLY-1023-YS199
Biotin-PEG4-amine NH2 COOH, CHO OLY-1023-YS204
Biotin-PEG11-amine NH2 COOH, CHO OLY-1023-YS207
Biotin Hydrazide Hydrazide COOH, CHO OLY-1023-YS184
Biotin-LC-hydrazide Hydrazide COOH, CHO OLY-1023-YS187
Biotin-PEG4-hydrazide Hydrazide COOH, CHO OLY-1023-YS205
6-Biotinamidohexanoic Acid COOH NH2 OLY-1023-YS190
Biotin-PEG4-propionic Acid COOH NH2 OLY-1023-YS191
Biotin-PEG3-acetic Acid COOH NH2 OLY-1023-YS217
  • Site-Specific Biotinylation Platform

With our unique in vivo biotinylation system, we can offer protein/antibody site-specific biotinylation services while eliminating heterologous antibody interference.

Creative Biolabs is a company that has long focused on the research and exploration of protein labeling technology. With a seasoned team of experts, we offer tailored protein-biotin conjugation solutions to meet the unique needs of your project. If you are interested in our services, please do not hesitate to contact us for a detailed description of our services and procedures. We look forward to collaborating with you!

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