Custom Protein-Photoaffinity Label Conjugation Service
As a seasoned expert in the field of bioconjugation, Creative Biolabs is committed to providing a variety of custom photoaffinity labeling (PAL) services to customers around the world. Our dedicated scientists have accomplished numerous challenging projects in this field. Our vision is to help you achieve great breakthroughs in your protein and antibody projects.
Overview of PAL
In recent years, photoaffinity labeling (PAL) has gradually emerged as a promising technique to study protein properties and functions. PAL is a method of binding chemical tags to protein molecules with very high tag specificity. The basic principle of PAL is to introduce probes into the system under study and convert them into highly reactive intermediates capable of covalently binding to biomolecules by irradiation with near ultraviolet or visible light. In this process, probes are often analogs of complex molecules in which certain functional groups are replaced with photoreactive groups, such as azide, diaziridine, or benzophenone. Furthermore, in order to detect cross-linked products, the probe must contain a reporter group. Unlike chemical tags, photoaffinity tags are free to form covalent bonds between ligands and their specific binding proteins.
Fig. 1 Basic principle of PAL.
Applications of PAL
PAL occupies a crucial position in protein-ligand interactions. PAL can recognize unknown targets of ligands and play a crucial role in elucidating protein function and conformational changes, as well as identifying new binding sites in proteins. In addition, PAL has been used to analyze a range of small molecule interactions, including drugs, lipids, metabolites, and cofactors. Most importantly, target protein identification is a critical step in drug discovery and PAL can be used to identify binding proteins in complex mixtures of biomolecules. By this method, it is feasible to identify the amino acid residues located at these sites to obtain structural information of the ligand binding site. Therefore, PAL is an attractive strategy for drug development.
Service at Creative Biolabs
Currently, Creative Biolabs offers a variety of customized PAL services. We are committed to uncovering protein-ligand interactions and protein-protein interactions (PPIs) in drug discovery by using many diaziridine-based probes. In particular, the corresponding diaziridine-based optical probes based on the linking of photoreactive groups with biological ligands, such as amino acids, nucleic acids, lipids, and carbohydrates, have been widely used in many fields, such as nucleic acid-protein interactions, lipid-protein interactions, carbohydrate-protein interactions, and steroid-protein interactions.
For your convenience, we deliver a one-stop PAL service that covers probe synthesis, product purification, characterization, and result analysis. In addition, we also welcome specific requests from our customers. Creative Biolabs delivers custom synthesis of innovative bioprobes to meet the unique requirements of your projects. If you are interested in our PAL services, please contact us for more details.
Related Sections