Custom Protein-Metal Chelates Conjugation (Radiolabel) Service
At Creative Biolabs, we offer custom protein-metal chelates conjugation services by covalently attaching proteins to metal chelate ligands that can be used as molecular imaging probes. With our advanced platform and decades of experience, every project is meticulously monitored according to strict quality control standards. Our vision is to help every client achieve breakthrough progress.
Overview of Protein-Metal Chelates Conjugation
Proteins can be labeled with metal elements so that they can be used as imaging reporter molecules or for other applications. The steps of this technique are simple. The target protein first binds to a suitable chelating agent to form a stable complex. Specifically, the protein was first desalted into a suitable buffer, then a bifunctional chelating agent was added, followed by desalting the protein conjugate into an acidic buffer. The conjugate is finally labeled by adding the appropriate metal salt. There are different requirements for chelators. First, the chelator is able to react with proteins without adversely affecting their chelating properties. In addition, chelators form stable complexes with target metals that do not dissociate in their biological environment.
Fig. 1 Schematic of metal chelate affinity precipitation of proteins.
Applications
Some metallic elements have properties that make them attractive in a variety of imaging modalities. These properties mainly contain fluorescence, paramagnetism and radioactivity. In recent years, these properties have gradually become the focus of exploring the function of biomolecules in biological systems. Protein metal chelates obtained through a new series of bifunctional chelators will be suitable for biomedical applications. The final products of metal ion conjugates play important roles in radioimmunoassay, radioimmunotherapy, magnetic resonance imaging and photodynamic therapy. For example, these products can be used as in vivo probes in biological systems and in vitro probes in analytical systems such as radioimmunoassays. Furthermore, novel metal chelate protein conjugates are promising tools in tumor diagnostic imaging and tumor therapy.
Our Services
At Creative Biolabs, the final produced labeled conjugates need to be characterized using various assays. The extent of protein aggregation was determined by high-performance liquid chromatography (HPLC) techniques and mass spectrometry was used to measure the number of chelating groups introduced. Importantly, our dedicated team will assess the biological function of the binding protein to ensure product quality. Additionally, Creative Biolabs offers a full line of high-quality custom bioconjugation reagent systems as well as custom synthesized and functionalized biomolecules ready for cross-linking. Our expertise and experience in protein and bioconjugation chemistry help our clients design the most suitable solution. We have delivered thousands of custom-conjugated biopolymers.
Please feel free to contact us to discuss your project plans and our dedicated scientists will design and develop suitable synthetic methods for your specific project.
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