Exosomal Surface Markers-based Exosome Characterization
Exosomes derived from cells under both normal and pathological conditions. Exosomes contain diverse types of proteins including common membrane and cytosolic proteins as well as origin-specific subsets of proteins reflective of cell functions and conditions. Empowered by leading technologies as well as years of experience in this field, Creative Biolabs has successfully launched an innovative exosome characterization platform for investigating the release of exosomes and exosomal markers value.
Why Exosome Characterization Is Important?
Exosomes are small cell-derived vesicles of 30–150 nm that are present in many and perhaps all biological fluids. Exosomes carry various molecular constituents with their origin cells, including proteins, lipids, mRNAs, and microRNAs (miRNAs). Depending on the cellular origin, exosomes recruit various cellular proteins that can be different from the plasma membrane including major histocompatibility complex molecules, annexins, flotillin, Alix, TSG101, integrins and tetraspanins (CD9, CD37, CD53, CD63, CD81, CD82 and CD151), and they are enriched in raft-lipids, such as cholesterol, ceramide and sphingolipids. Recently, an increasing number of exosomal proteins have been found to be potential biomarkers for a variety of diseases including cancer as well as liver and kidney diseases. It is essential to investigate the release of exosomes from cell and characterize the exosomal markers. This can be achieved using a panel of routine techniques such as transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), multi-colour flow cytometry, ELISA and Western blotting, as used in the present study.
Fig.1 Composition of exosomes.1,2
Exosomal Surface Markers-based Exosome Characterization Services
Exosomes present novel therapeutic strategies. Therefore, develop exosome characterization is required for investigating the release of exosomes and exosomal markers value. Creative Biolabs offers robust services to fulfill the demands.
1) Exosome Characterization via FACS
Using an optimally configured FACS flow cytometer, we provide service to analyse and sort exosomes based solely on the presence of endogenous membrane constituents, including EGFR and the exosomal marker, CD, and so on.
2) Exosome Characterization via Western Blotting
As there are no exosome specific markers, proteins enriched in exosomes, from all different cellular origins, are commonly used for exosome detection. Thus, depending on the origin cells the markers for detection may vary. Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture.
3) Exosome Characterization via ELISA
ELISA is a patented double sandwich ELISA assay for quantitative and qualitative analysis of exosomes. The quantification and characterization of exosomes proteins are followed by the use of appropriate detecting antibodies to detect the exosomes associated antigens, which can be generic or cell-specific exosomes.
Features of Our Services
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Highly efficient
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No investment for instrument
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Feasible for multiple marker analysis
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Best after-sale service
With the help of our well-established technologies and experienced scientists, Creative Biolabs provides customized exosome characterization service for customer samples. We provide very flexible options for each specific case, making it accessible to all kinds of research and industrial customers. Besides, we are open to discussions. Please contact us for more information.
References
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Gurung, S.; et al. The exosome journey: from biogenesis to uptake and intracellular signalling. Cell Communication and Signaling. 2021, 19(1):47.
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under Open Access license CC BY 4.0, without modification.
For Research Use Only. Cannot be used by patients.
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