Western Blotting based Exosome Characterization Service
Overview Services Features FAQs
Overview
Western Blot is a widely used experimental method in molecular biology, biochemistry, and immunogenetics. Leveraging years of experience in exosome characterization, Creative Biolabs has developed an innovative exosome characterization platform based on Western blotting.
Western Blotting
Western blotting, also known as immunoblotting, is a powerful technique for identifying and quantifying specific proteins within complex mixtures. Due to its relatively low cost, high sensitivity, and flexibility, Western blotting is extensively used in research laboratories worldwide to measure protein levels. The availability of commercially produced antibodies has further popularized this technique in the life sciences. Currently, the recognized specific marker proteins for exosomes include CD9, CD63, CD81, and TSG101. Additionally, exosomes from different cellular sources are enriched with marker proteins consistent with their donor cells.
Fig. 1 Results of detecting exosome markers (TSG101, CD63, and CD9) using Western Blotting.
Services
Our general workflow for Western blotting analysis includes a specialized protein isolation method prior to the blotting process. We assist in selecting suitable antibodies, and our highly experienced expert team ensures quality management at every step, delivering confident and unbiased results.
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Sample Preparation
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Gel Electrophoresis
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Transfer
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Blocking
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Antibody Detection
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Signal Detection
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Data Analysis
Fig. 2 Complete western blotting apparatus setup.
Features
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Special methods designed for exosome protein isolation
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High quality pictures with low background and bright bands
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Abundant antibody resources
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Skillful scientific team
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Best after-sale service
The Western Blot method is very mature, the most lab has equipment to finish it. However, getting good results requires a lot of time and effort for optimization. With the help of our well-established technologies and experienced scientists, Creative Biolabs has special skills in exosome protein isolation, transprinting and antibody dilution. We customize the experiment plan for customer samples. We have abundant antibody resources to meet requirements of different sources exosome, and provide flexible options for each specific case. We are happy to make it accessible to all kinds of research and industrial customers. Please don't hesitate to contact us for more information.
FAQs
Q: Which exosome marker proteins can Western Blotting detect?
A: We can provide five antibody markers (CD9, CD63, CD81, TSG101, Calnexin) that react with exosomes derived from humans, mice, and rats. Detection of CD9, CD63, CD81, TSG101, Calnexin in exosomes from other species or testing other antibody markers requires customers to provide their own antibodies or cover additional costs for antibody procurement.
Q: How much sample is required for this service?
A: If quantifying by particle number, at least 40μL per marker per sample should be provided, with an exosome concentration of ≥10^10 particles/mL; if quantifying by BCA, at least 10μg per marker per sample should be provided, with an exosome concentration of ≥0.25μg/μL. As exosomes from different sources can vary, we recommend that customers provide as much sample as possible.
Q: What should I do if the results do not meet expectations?
A: Our team will provide comprehensive technical support to help analyze possible reasons and optimize experimental steps in the next repetition. We commit to closely collaborating with our customers to ensure your research needs are met.
For Research Use Only. Cannot be used by patients.
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