Exosome labeling is a novel strategy for monitoring exosome trafficking in vivo, which contributes to optimizing exosome-based diagnosis and treatment. With years of experience in exosome labeling, Creative Biolabs has developed fluorescent labeling and biotin labeling techniques to track the distribution and movement of specific exosomes.
Exosomes are small vesicles (30-150 nm in diameter) containing sophisticated RNA and protein cargos and function as a vector of intercellular communication. To further our understanding of exosome characteristics, an exosome labeling strategy is raised to investigate the biology and distribution of exosomes, and monitor exosome trafficking in real-time in vivo without invasion. This technique is a useful tool for the advancement and improvement of exosome-based therapeutics and diagnosis.
Fig.1 Detection of extracellular vesicles by flow cytometry with calcein AM.1
Here, we provide two technological platforms for exosome labeling.
Antibodies labeled with fluorescent dyes have a high affinity with the specific cell-of-origin antigens on the surface of exosomes. Proteins on exosomes can be directly labeled with fluorescent dyes. Therefore, exosomes labeled with fluorescent dyes can be used to track and visualize particular exosome components or to follow the real-time transport of exosome cargo to cells.
With its binding partners, avidin and streptavidin, biotin is a tiny molecule that creates one of the strongest non-covalent interactions observed in nature. Protein labeling with biotin is a helpful technique for processes like exosome purification and detection because of the extremely strong non-covalent interaction. To identify exosome trafficking in vivo or in model animals, for instance, exosomes containing certain proteins tagged with biotin are employed. Importantly, biotin is a desirable choice for a label because of its small size, which rarely interferes with protein action.
A: Exosome labeling is the process of attaching a fluorescent dye or other marker to exosomes to track and study their behavior in vitro or in vivo.
A: Exosome labeling is important because it allows researchers to visualize and track exosomes in real-time, enabling a better understanding of their functions and potential applications in diagnostics and therapeutics.
A: There are several labeling techniques available for exosomes, including fluorescent dyes, biotin/streptavidin systems, and nanoparticles. Each method has benefits and drawbacks that vary based on the objectives of the study.
A: In general, exosome labeling should not significantly alter the biological properties of exosomes. However, it is important to carefully select the labeling technique and optimize the labeling conditions to minimize any potential effects on exosome function.
Exosome labeling in Creative Biolabs acts as an effective tool for the investigation of exosome characteristics and exosome trafficking for therapeutic and diagnostic applications. If you are interested in exosome labeling, please contact us for more information whenever you feel free.
Reference