Exosomes can be effectively loaded with therapeutic medications, including synthetic oligonucleotides, small molecule chemicals, and viral vectors, as multiple investigations have demonstrated. To maximize exosome loading technology and raise production efficiency, Creative Biolabs offers exosome cargo loading services.

Overview

• Exosomes are small extracellular vesicles that play a critical role in intercellular communication. They can be engineered to transport a variety of payloads, such as tiny compounds, RNAs, and proteins.
• Exosome cargo loading services are designed to optimize the delivery of these agents for research applications. Our services encompass the entire spectrum of exosome cargo loading, from the isolation and purification of exosomes to the precise loading of desired cargo.
• Our expertise extends to customizing exosomes for targeted delivery, enhancing the therapeutic potential of the cargo.

Cargo Loading Services in Creative Biolabs

Proteins - In addition to loading with small molecules, a key component of our exosome engineering services involves loading with large molecules such as proteins. It is possible to tailor and target the proteins onto the surfaces of exosomes, allowing for tissue targeting and surface display of proteins. Besides, proteins can also be loaded inside the exosomes for therapeutic delivery, leveraging both the natural properties of exosomes and the specificity of biological molecules.

Nucleic acids - Small interference RNA (siRNA), miRNA, short hairpin RNA (shRNA), and other nucleic acids can be incorporated into exosomes using different strategies. Numerous studies have employed exosomes from different sources as carriers of small RNAs to treat various diseases, especially brain diseases and tumors.

Several distinct approaches can be utilized at Creative Biolabs for cargo loading into exosomes:

• Post-loading Strategy

Through the processes of electroporation, lipofection, sonication, and calcium chloride, exosomes can be directly loaded with exogenous nucleic acids or medications.

• Pre-loading Strategy

Loading the foreign payload into parental cells for release into exosomes.

Fig. 1 Strategies for exosome drug loading.¹

Features of Cargo Loading Services in Creative Biolabs

• Customization: Ability to tailor cargo loading based on specific therapeutic needs.
• Efficiency: High loading efficiency ensures maximum cargo delivery.
• Stability: Maintaining cargo stability during the loading process to preserve efficacy.
• Versatility: Accommodation of various cargoes including RNA, DNA, proteins, and small molecules.
• Scalability: Capability to scale production for further applications.

Backed by our expert scientists and technology platform, Creative Biolabs can provide custom-specific cargo-loading services for proteins, nucleic acids, and small molecules of interest. Please contact us to discuss your project requirements.

FAQ

Reference

1. Zeng, Haifeng, et al. "Current Strategies for Exosome Cargo Loading and Targeting Delivery." Cells 12.10 (2023): 1416. Under Open Access license CC BY 4.0. The image was modified by revising the title.

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