CRISPR Interference (CRISPRi) Screening Service
CRISPRi is a gene silencing method that allows precise and reversible repression of gene expression without modifying the DNA. Compared to RNA interference, CRISPRi offers greater specificity and fewer off-target effects, making it ideal for loss-of-function studies. It is especially useful for analyzing essential genes, identifying pathway suppressors, and validating drug targets. Creative Biolabs delivers a complete CRISPRi screening service, featuring high-coverage sgRNA libraries, efficient lentiviral delivery, and robust data analysis to support accurate gene function discovery and target identification.
Introduction of CRISPR Interference (CRISPRi) Screening Service
CRISPR Interference (CRISPRi) is a powerful transcriptional repression technique that enables targeted downregulation of gene expression without introducing any changes to the DNA sequence. This approach employs a nuclease-deactivated Cas9 (dCas9) coupled with transcriptional repressor domains like KRAB to inhibit gene expression. Guided by sequence-specific sgRNAs, the dCas9-repressor complex binds to promoter or regulatory regions of target genes, thereby blocking transcription initiation or elongation and effectively silencing gene activity. CRISPRi has become an essential tool for loss-of-function studies, offering higher specificity and fewer off-target effects than traditional RNAi approaches. It avoids the complications of partial knockdown and immune activation, making it particularly valuable for studying essential genes, mapping regulatory pathways, and discovering gene suppressors in disease models. Genome-wide CRISPRi libraries are designed to target nearly all protein-coding genes, typically using 4–6 sgRNAs per gene, allowing for systematic interrogation of gene function at scale and enabling researchers to identify genes involved in phenotypes such as drug response, cell fitness, and stress resistance.
Workflow of CRISPR Interference (CRISPRi) Screening Service
Fig. 1 Workflow of our CRISPRi screening service.
Advantages of CRISPR Interference (CRISPRi) Screening Service
- Extensive sgRNA Libraries for CRISPRi - Our sgRNA collections are designed to target nearly all protein-coding genes, with multiple guides per gene to ensure strong and reliable gene knockdown.
- Flexible Screening Formats - We provide adaptable CRISPRi screening setups, from full-genome repression libraries to pathway- or function-specific sub-libraries, aligning with diverse experimental goals and study models.
- Optimized Lentiviral Delivery for Consistent Gene Suppression - Our refined lentiviral platform enables efficient and stable delivery of dCas9-KRAB constructs and sgRNAs, ensuring durable transcriptional repression across a variety of cell types.
- Strict Quality Standards & Deep Data Insight - Each phase is rigorously monitored. Our advanced computational pipeline delivers high-confidence gene suppression profiles and phenotype-linked insights.
- Affordable and Reliable Partnership - We offer cost-effective service packages backed by expert support, helping clients navigate CRISPRi projects efficiently and with confidence from initiation to data delivery.
How CRISPR Interference (CRISPRi) Screening Service Can Assist Your Project
At Creative Biolabs, our CRISPRi screening service follows a meticulously optimized workflow, ensuring precision, reproducibility, and data reliability at every stage. From sgRNA library preparation and lentiviral packaging to cell transduction, screening, and sequencing, each step is rigorously quality-controlled to deliver consistent and high-confidence results. By precisely silencing gene expression at the transcriptional level, our CRISPRi platform empowers researchers to identify essential genes, disease pathway regulators, and drug response modulators. Through advanced bioinformatics analysis, we translate screening data into actionable insights, mapping gene knockdown effects to phenotypic changes, suppressor gene identification, and novel therapeutic targets.
Whether your focus is on pathway analysis, target validation, or synthetic lethality studies, CRISPRi screening service of Creative Biolabs delivers high-resolution functional data to accelerate your discoveries. Contact us today to explore how our tailored CRISPRi solutions can enhance your research and drive impactful results.
