CRISPR assisted Library Design Service
Custom CRISPR library design involves the tailored selection and synthesis of guide RNAs (gRNAs) targeting specific genes, pathways, or genomic elements of interest, enabling researchers to perform highly focused and efficient functional screens. These libraries can be scaled to fit various project sizes, from compact sub-libraries to large-scale pooled formats, depending on the research scope, throughput needs, and cell model system. At Creative Biolabs, we specialize in designing CRISPR libraries that are optimized for various screening applications.
Introduction of Custom CRISPR Library Design Service
Custom CRISPR library design service involves the creation of guide RNA (gRNA) sets specifically designed to target user-selected genes or genomic regions for functional screening using CRISPR technology. This strategy enables researchers to systematically interrogate gene functions, uncover regulatory networks, and identify therapeutic targets with high specificity. Unlike pre-made commercial libraries, which often include genome-wide or broad-content collections, custom libraries provide the advantage of target-focused design, allowing researchers to narrow down on pathways, gene families, or disease-relevant loci of interest. In addition, the flexibility in library size, from compact, pathway-specific panels to large-scale pooled formats, makes them suitable for a wide range of screening scales and experimental systems. By tailoring the gRNA content and optimizing design parameters such as on-target efficiency and minimal off-target potential, custom CRISPR libraries offer improved performance, cost-effectiveness, and clearer data interpretation, making them a valuable asset in both discovery science and translational research.
Advantages of Custom CRISPR Library Design Service
Fig. 1 Advantages of custom CRISPR library design service.
- Target-Specific Design - Focuses on user-defined genes or pathways, avoiding unnecessary content present in genome-wide commercial libraries and enhancing data relevance.
- Flexible Library Scale and sgRNA Number - Libraries can be tailored in both total size and the number of sgRNAs per gene, allowing researchers to balance coverage and screening throughput according to project needs.
- Higher Experimental Efficiency - Optimized gRNA selection improves on-target activity and reduces off-target noise, leading to cleaner screening outcomes and more actionable results.
- Cost-Effective Strategy - Reduces reagent usage and data analysis burden by limiting the screen to relevant targets, maximizing impact while minimizing unnecessary expense.
- Comprehensive Design Support - Creative Biolabs provides expert assistance throughout the process, from gRNA design to vector construction and library synthesis, ensuring precision and reliability.
What We Can Do
At Creative Biolabs, we bring extensive experience in CRISPR library screening, with deep technical understanding at every step, from gRNA design to data interpretation. Our workflows are supported by a rigorous quality control system, ensuring that each element of the process meets high standards for reliability and reproducibility. We tailor our services to meet your specific research needs.
For clients who wish to conduct their own screening but require a well-constructed library, we offer:
For clients who need compatible cellular models for various kinds of screening, we can provide:
- Cas9 Overexpressing Cell Line Development
- dCas9-VPH Overexpressing Cell Line Development
- dCas9-VPR Overexpressing Cell Line Development
- dCas9-KRAB Overexpressing Cell Line Development
We also offer a fully integrated screening service, covering:
- Genome-Wide CRISPR Screening
- CRISPRa Screening
- CRISPRi Screening
- Single-Cell CRISPR Screening
- Custom CRISPR Screening
Whether you're looking for specific components or an end-to-end CRISPR screening solution, Creative Biolabs is ready to support your project from start to finish. Contact us to explore how our customized services can help accelerate your discoveries.
