Nuclease Activity Measurement Service

Nucleases are enzymes that play a critical role in nucleic acid metabolism by cleaving the phosphodiester bonds within DNA or RNA molecules, whose function is essential in various biological processes. Accurate measurement of nuclease activity is crucial for understanding enzyme functionality and ensuring the success of therapeutic and diagnostic applications involving nucleic acid manipulation. To support these nuclease-related research and development efforts, Creative Biolabs offers specialized nuclease activity measurement services. With advanced technologies and extensive expertise, we assist researchers and clients worldwide in generating reliable data for their enzyme characterization studies.

Introduction of Nuclease Activity Measurement Service

Nucleases are a diverse group of enzymes that degrade nucleic acids by hydrolyzing the phosphodiester bonds within DNA or RNA molecules. They are fundamental to many biological pathways. In both basic research and applied fields such as gene therapy, diagnostics, and biotechnology, understanding the precise behavior of nucleases is essential for ensuring their effectiveness. Nuclease activity measurement serves as a critical step in characterizing these enzymes. It allows researchers to determine how a nuclease interacts with various nucleic acid substrates whether it exhibits sequence or structure-specific preferences, and how its activity is influenced by environmental factors such as ion dependence and temperature. In addition, for programmable systems like CRISPR, it is important to assess collateral cleavage activity, which refers to unintended cutting of non-target nucleic acids after activation. Through comprehensive measurement and profiling, researchers can gain detailed insights into the biochemical properties and functional potential of nucleases. This information is crucial for enzyme engineering, selection of appropriate tools for molecular applications, and the development of novel genome-editing technologies or nucleic acid-based detection systems.

Fig. 1 Nuclease activity measurement of Fanzor protein.¹

Advantages of Nuclease Activity Measurement Service

• Comprehensive Characterization - Allows in-depth analysis of nuclease properties and accurately profiles substrate specificity, ion dependence, temperature range, and collateral cleavage.
• Application-Driven Insight - Helps assess enzyme suitability for in real-world applications and enhances experimental design like gene editing, diagnostics, and therapeutic use.
• Support for Optimization - Detailed activity data aids in modifying nucleases for higher stability, specificity, or efficiency and efficient candidate screening.
• Reliable Results from Proven Platform - Backed by extensive experience and robust assay systems, ensuring consistent and trusted data.
• Cost-Effective and Client-Focused - Offers flexible, affordable services with responsive technical support tailored to project needs.

What We Can Do

With a well-established experimental platform and extensive hands-on experience, we specialize in comprehensive characterization of nuclease activity. Our team is adept at designing and executing precise assays to assess key parameters such as substrate preference (ss/dsDNA, ss/dsRNA), metal ion dependence, temperature adaptability, and potential collateral cleavage behavior. Through rigorous testing and optimized protocols, we deliver high-quality, reliable data that provide deep insights into nuclease function. These results can be used to anticipate enzyme performance under real-world conditions, refine experimental setups, and guide the selection of suitable nucleases for applications in gene editing, molecular diagnostics, or therapeutic development.

Whether you're exploring a new nuclease or optimizing an existing system, Creative Biolabs is ready to support your research with expert analysis and actionable data. Contact us to discuss your project and learn how we can accelerate your discovery process.

Reference

1. Saito, Makoto, et al. "Fanzor is a eukaryotic programmable RNA-guided endonuclease." Nature 620.7974 (2023): 660-668.
   Distributed under Open Access license CC BY 4.0. The image was modified by extracting and using Part D-G of the original image.