Gene Knock-in Service

Knock-in (KI) involves the precise insertion of specific genes or sequences into defined genomic sites, allowing the introduction of reporter genes or therapeutic genes under natural gene regulation. This is essential for studying gene function, tracking expression, and developing disease models. Creative Biolabs offers high-quality CRISPR knock-in cell line services with reliable, precise gene integration, helping global clients create stable, customized models to advance their research.

Introduction of Gene Knock-in Service

Gene KI is a genetic engineering technique that precisely inserts specific genes or sequences into designated genomic locations. Unlike knockout, which removes gene function, knock-in introduces new elements—such as reporter genes, fluorescent tags, or therapeutic genes—while preserving the overall integrity of the genome. This approach is widely used to study gene expression, protein localization, and regulatory mechanisms in a controlled cellular environment. By enabling stable and accurate gene integration, KI cell models play a critical role in disease modeling, drug discovery, and functional genomics, making them an essential tool in modern biological research.

Workflow of Gene Knock-in Service

Fig. 1 Workflow of our gene knock-in service.

Advantages of Gene Knock-in Service

• High Success Rate & Stability - Efficiently integrates sequences up to 2000 bp with high precision, ensuring stable and reproducible results.
• Precise Disease Modeling - Introduces disease-related genes to create reliable models, overcoming the limitations of scarce patient samples.
• Broad Cell Type Adaptability - Works with various cell types, including stem and primary cells, supporting diverse research applications.
• Strict Quality Control - Validates knock-in accuracy and stability through PCR, sequencing, and other rigorous testing methods to guarantee reliable results.
• Affordable & Reliable Support - Provides cost-effective solutions with dedicated after-sales assistance for seamless project execution.

How Gene Knock-in Service Can Assist Your Project

Creative Biolabs is dedicated to delivering high-quality knock-in (KI) single-cell clones with precise gene integration, stable expression, and long-term reliability. Our advanced CRISPR-based KI technology ensures accurate insertion while maintaining the natural gene regulation within cells, making these models ideal for studying gene function, protein expression, and disease mechanisms. Each clone undergoes rigorous quality control, including PCR sequencing and mycoplasma testing, to verify successful gene integration, eliminate contamination risks, and ensure consistent, reproducible results. Additionally, multiple clients have confirmed the effectiveness of our KI cell lines through independent functional assays, demonstrating their reliability in real-world applications.

By providing customized, contamination-free KI cell models, Creative Biolabs help researchers obtain more precise, reproducible data, accelerating discoveries in biomedical research and drug development. Contact us to explore how our KI cell line services can enhance your research projects.