In Vivo Screening Introduction

Phage display library construction and phage library screening are important steps in phage display technology, and they are crucial for the results of phage display technology. Currently, there are two novel methods for phage library screening - in vivo screening and ex vivo screening, which have their advantages and disadvantages. In specific experiments, the appropriate method can be selected according to the actual research needs and experimental program. Based on our rich practical experience and research platform, Creative Biolabs provides comprehensive services and technologies for in vivo phage display.

Phage. (Creative Biolabs Authorized)

In vivo Phage Display

The successful application of antibody libraries cannot be separated from library screening technology. in vivo screening is one of the important screening methods. Since many living cells are difficult to culture in vitro and in vitro culture may change the cell membrane state, the specificity of phage antibodies observed in vitro may not be obvious or exist in vivo, so in vivo biopanning is of special significance.

Intact monoclonal antibodies are not suitable for in vivo biopanning because of their high molecular weight and their tendency to elicit immune response. The most commonly used method is to inject a small molecule antibody fragment library into the tail vein and rely on blood circulation to deliver antibodies to the whole body. After a period of circulation, the non-specifically bound molecules will be gradually diluted with the circulation and eventually cleared, and the vascular epithelial tissues of different tissues and organs can be isolated, and the tissue-specific antibody can be obtained by biopanning of the phage and amplification. Some scholars have screened specific phage antibodies against endothelial cells in the mouse thymus.

Because biopanning is performed in vivo, it is not possible to seal on specific sites. In addition, blood flow has a complicated effect on the binding and storage of phage and vascular epithelial cells, and the binding time of antibody libraries is too short for specific organs, so the diversity advantage can't be fully realized, which leads to the arbitrariness of the results. Some scholars first immunized mice with isolated epithelial cells to construct immune antibody libraries and then eluted the antibody libraries by immobilizing cell membranes, which integrated the advantages of in vivo immunization and in vitro screening and could either pre-clean the phage libraries with the help of negative selection or optimize the specificity of selection by optimizing the conditions of incubation and washing.

Creative Biolabs has a wealth of knowledge and experience in antibody biopanning. We are happy to share our knowledge and experience in the fields of antibody biopanning and phage display library screening with you.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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