Catharanthus roseus-derived Exosome Research and Application

The scientific questions surrounding how plant exosomes are formed, how they are characterized, and how they are prepared in large quantities are major challenges for researchers. Creative Biolabs has established an excellent technology platform for the development of plant exosomes and is able to provide pilot research services on exosomes from a variety of flower sources, including Catharanthus roseus.

Catharanthus roseus-derived Exosome (CRDE) Isolation

Collect fresh Catharanthus roseus leaves and wash them to remove dirt.
Digest the leaves with cellulase and pectinase.
Centrifuge to remove protoplasts and collect supernatant.
Centrifuge to remove plant fibers and collect supernatant
Concentrate the supernatant with hollow fiber modules
Isolate nanovesicles from concentrated samples using the sucrose buffer-ultracentrifugation method
Remove sucrose using ultrafiltration tubes, resuspend exosomes, and store frozen until use.

Research on CRDE

Research item	Conclusion
CRDE characterization	CRDE has excellent stability, is undamaged by nuclease and protease digestion, maintains membrane vesicle morphology after acid/base treatment, and is resistant to acid/base environments and enzyme treatments.
CRDE biodistribution	CRDE can stay in immune organs after intraperitoneal injection and can be effectively absorbed by the stomach and anti-gastric acidity after oral administration. While CRDE after intravenous injection is rapidly captured and cleared by the liver and spleen.
CRDE component profiling	Lipidomics analysis reported high levels of ether phospholipids in CRDE, and this particular lipid composition may be potentially relevant to the stability and immunomodulatory activity of CRDE.
	Metabolomics analysis demonstrated the therapeutic potential of CRDE for immunodeficiency diseases.
	Differential proteomics analysis identified 6 CRDE marker proteins. Significantly enriched polycystin-associated proteins were identified for the first time in CRDE, indicating the possibility of CRDE originating from MVB.
CRDE stimulate immunity mediated through the TNF-α/NF-κB/PU.1 axis	In vitro pharmacological experiments evaluated that CRDE significantly stimulated TNF-α secretion from macrophages, agonized the NF-κB signaling pathway, upregulated the hematopoietic-related transcription factor PU.1, and promoted the proliferation of primary mouse spleen lymphocytes.
CRDE relief immunosuppressive effects	The results of animal experiments showed that CRDE can significantly alleviate the immunosuppressive effects induced by cyclophosphamide, and has potential application in the treatment of immune system diseases.
Feasibility study of CRDE scale-up production	A Catharanthus roseus leaf dedifferentiated cell system and a Catharanthus roseus stem-forming layer stem cell system were established by plant cell culture technology, which could achieve more than 1 g of nanovesicles from tens of liters of supernatant of suspension-cultured dedifferentiated cells, and greatly increased the yield of CRDE. This initially demonstrated the feasibility of scaled-up production of CRDE.

Fig. 1 Isolation and analytical studies of CRDE.¹

CRDE Applications

CRDE can mimic liposomes in the digestive system and remain stable in the environment of strong acids and bases and enzymatic digestion, which can be used as an ideal tool for drug delivery.
CRDE possess immunomodulatory activity, which make the treatment more effective in attacking tumor cells and have potential applications in tumor therapy.
CRDE also have potential in other areas of application, such as as carriers for nutritional supplements or food additives, and as cosmetic additives to deliver active substances for skin care.

The exploration and understanding of plant-derived exosomes, including CRDE, is advancing, providing an experimental basis for their application in areas such as disease therapy and drug delivery. In addition, the identification of universal marker proteins for plant exosomes provides a feasible basis for their engineering. Creative Biolabs specializes in exosome research and provides feasibility testing and development services for in-depth studies of a wide range of plant exosomes, including CRDE. Please contact us to learn more.

Comprehensive Catharanthus roseus-Derived Exosome Development Services at Creative Biolabs

Catharanthus roseus-Derived Exosome Isolation

Catharanthus roseus-Derived Exosome Identification

High-Throughput Screening Analysis (Proteins, RNA, Lipids and Metabolites)

Target Analysis

In Vitro Functional Discovery of Plant-Derived Exosomes

In Vivo Functional Discovery of Plant-Derived Exosomes

Large-Scale Production of Plant-Derived Exosomes

Reference

Ou, Xiaozheng, et al. "Novel plant-derived exosome-like nanovesicles from Catharanthus roseus: preparation, characterization, and immunostimulatory effect via TNF-α/NF-κB/PU. 1 axis." Journal of Nanobiotechnology 21.1 (2023): 1-22.

Plant Exosome Isolation