Tea Flower-derived Exosome Research and Application

Tea flower-derived exosomes have been found to be naturally rich in polyphenols, flavonoids, and functional fractions, with anti-inflammatory and anticancer potential in research and development of intervention strategies for a wide range of diseases. Creative Biolabs has accumulated insights related to tea flower-derived exosomes and can provide specialized services to advance the tea flower-derived exosome research project.

Tea Flower-derived Exosome Isolation

Blender chopping of tea flowers with PBS buffer.
Centrifuge the tea flower juice at low speed to remove large tea flower tissues and cellular debris.
Ultracentrifugation to collect the exosome precipitates of tea flower origin and resuspend with PBS.
Further centrifugation of the resuspension solution through sucrose density gradients to obtain purified tea flower exosomes.
Wash tea flower exosomes and freeze storage.

Fig. 1 Isolation and purification of exosomes derived from tea flowers.¹

Introduction to Tea Flower-derived Exosome

Features	Functions
Structural Stability Stability assessment of tea flower-derived exosomes has shown that their morphology and structure are stable under a variety of in vivo environmental simulants and media conditions, allowing for the administration of tea flower exosomes in various ways, including intravenously and orally. This facilitates their application in biology and medicine. Rich in various active substances Tea flower-sourced exosomes are rich in active substances such as anticancer polyphenols, flavonoids, proteins, and lipids, which exert multiple roles in living organisms, such as antioxidant, anti-inflammatory, antimicrobial, anticancer, and so on.	Can be internalized by tumor cells Tea flower exosomes can be internalized by tumor cells and effectively penetrate the intestinal epithelial layer of mice into the circulation, which offers the possibility of their application in drug delivery and therapy. Anti-cancer effects It is found that tea flower exosomes can stimulate the increase of oxidative stress in cancer cells, and induce mitochondrial damage, cell cycle arrest, and apoptosis, thus inhibiting the proliferation, migration, and invasion of breast cancer cells.

Features

Functions

Structural Stability
Stability assessment of tea flower-derived exosomes has shown that their morphology and structure are stable under a variety of in vivo environmental simulants and media conditions, allowing for the administration of tea flower exosomes in various ways, including intravenously and orally. This facilitates their application in biology and medicine.
Rich in various active substances
Tea flower-sourced exosomes are rich in active substances such as anticancer polyphenols, flavonoids, proteins, and lipids, which exert multiple roles in living organisms, such as antioxidant, anti-inflammatory, antimicrobial, anticancer, and so on.

Can be internalized by tumor cells
Tea flower exosomes can be internalized by tumor cells and effectively penetrate the intestinal epithelial layer of mice into the circulation, which offers the possibility of their application in drug delivery and therapy.
Anti-cancer effects
It is found that tea flower exosomes can stimulate the increase of oxidative stress in cancer cells, and induce mitochondrial damage, cell cycle arrest, and apoptosis, thus inhibiting the proliferation, migration, and invasion of breast cancer cells.

Applications of Tea Flower-derived Exosomes

Drug Carrier
Due to their biological activity and structural stability, tea flower exosomes can be applied as drug carriers, whereby drugs are encapsulated in tea flower exosomes through specific drug loading techniques in order to deliver the drug more efficiently to the target cells or tissues. This could increase the efficacy of the drug, reduce side effects, and improve therapeutic outcomes.
Intestinal Flora Regulator
Animal experiments have shown that tea flower exosomes can accumulate in mammary tumors and lung metastasis, inhibit tumor growth and lung metastasis, and regulate the structure of intestinal flora in mice. Therefore, tea flower exosomes may be able to be employed as intestinal flora regulators to improve the intestinal microbial environment of organisms, and then treat or prevent some diseases related to intestinal flora.
Cell Therapy
Since there has been research evidence that tea flower exosomes can be internalized by tumor cells, they may be suitable for the development of new cellular therapies. For example, combining tea flower exosomes with immune cells could potentially serve to specifically target and destroy tumor cells, thus providing a new strategy for tumor therapy.
Nutritional Supplements and Health Products
Since tea flower exosomes are rich in active substances such as polyphenols, flavonoids, proteins, and lipids, these substances have multiple benefits in maintaining human health. Therefore, tea flower exosomes can be developed as ingredients in nutritional supplements or health products to help maintain human health.

Fig. 2 Potency of tea flower exosomes for administration and induction of apoptosis in cancer cells.¹

Tea flower-derived exosomes offer a wide range of unique biological activities and application potentials, providing new research and development possibilities in the biomedical field. Creative Biolabs features a specialized exosome research platform and a technical team that is capable of providing customized research services related to tea flower exosomes. Please contact us to advance your tea flower exosome research.

Reference

Chen, Qiubing, et al. "Natural exosome-like nanovesicles from edible tea flowers suppress metastatic breast cancer via ROS generation and microbiota modulation." Acta Pharmaceutica Sinica B 12.2 (2022): 907-923.

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