The circRNAs in exosomes have been widely indicated to be closely involved in tumor cell proliferation and tumor invasion. Creative Biolabs is committed to providing top-notch and reasonably priced exosomal circRNA sequencing services to advance the identification of tumor biomarkers.

Exosomal circRNA & Its Sequencing

The expression and abundance of circRNAs vary in a tissue- or cell-specific manner. Human transcriptomes have been found to include a large number of circRNAs, particularly in serum exosomes. Using RNA sequencing techniques, it was discovered that exosomes had higher concentrations of circRNAs than the parent cells did. Moreover, exosomal circRNAs have a variety of biological roles, such as but not restricted to:

• Act as miRNA sponges, regulating the miRNA target gene expression.
• Bind to RNA binding proteins.
• Promote or inhibit cell proliferation.
• Contribute to tumor cell survival, invasion, and metastasis.

Fig.1 Biological functions of exosomal circRNAs.¹

Exosomal circRNAs have been demonstrated in studies to be essential for the growth of tumor cells. Moreover, the dysregulated circRNAs in the exosome may also alter the tumor microenvironment and associate with chemotherapy drug resistance. These important actions in tumor development, together with their stable circular structure, abundance, and tissue specificity, endow exosomal circRNAs with huge potential as new biomarkers for the diagnosis of human tumors.

Powerful high-throughput RNA sequencing is the preferred technology for exosomal circRNA profiling and biomarker identification. The seasoned next-generation sequencing of exosomal circRNA from specific tissues or fluids can provide a series of important information, such as:

• Profiling known or target circRNAs under specific or diseased conditions.
• Identifying novel circRNAs.
• Identifying circRNAs biomarkers for disease classification and diagnostics.
• Exploring the circRNAs networks and function mechanisms.

Fig.2 The expression landscape of circulating exosome-derived circRNAs in GBM patients.²

For Exosomal circRNA Sequencing Services, Choose Creative Biolabs

Our team of experts specializes in exosomal circRNA research, offering comprehensive sequencing services backed by extensive bioinformatics support, to facilitate your multifaceted research applications as below.

• Biomarker Discovery: Identify exosomal circRNAs associated with disease states or physiological conditions.
• Functional Studies: Investigate the roles of exosomal circRNAs in cellular communication and disease progression.
• Therapeutic Potential: Explore exosomal circRNAs as potential targets for therapeutic interventions.

Fig.3 Workflow of exosomal circRNA sequencing.

Features

• Comprehensive Exosomal circRNA Profiling: Utilize state-of-the-art sequencing technologies to comprehensively profile exosomal circRNAs.
• High Sensitivity and Specificity: Achieve high sensitivity and specificity in detecting exosomal circRNAs, ensuring accurate and reliable results.
• Expert Bioinformatics Support: Benefit from expert bioinformatics support to interpret data and derive meaningful biological insights.
• Quick Turnaround Time: Quick turnaround times are guaranteed via effective workflows without sacrificing the quality of the data.

Please feel free to contact us for more information about our comprehensive sample preparation requirements and quotes for our exosomal circRNA sequencing service at Creative Biolabs.

FAQs

References

1. Seimiya, Takahiro, et al. "Emerging roles of exosomal circular RNAs in cancer." Frontiers in cell and developmental biology 8 (2020): 568366. Under Open Access license CC BY 4.0. The image was modified by revising the title.
2. Xia, Dongyan, and Xuhui Gu. "Plasmatic exosome-derived circRNAs panel act as fingerprint for glioblastoma." Aging (Albany NY) 13.15 (2021): 19575. Under Open Access license CC BY 3.0, without modification.

• Exosomal Small RNA/miRNA Sequencing
• Exosomal lncRNA Sequencing
• Exosomal mRNA Sequencing
• Exosomal mRNA Microarray
• Exosomal miRNA Microarray
• Exosomal LncRNA Microarray
• Exosomal ceRNA Microarray
• Exosomal Whole Transcriptome Sequencing