Exosomal miRNA has been confirmed as a nonnegligible player in the initiation and progression of tumors and cancers. To accelerate the exploration of the role of exosomal miRNA in cancers and the identification of exosomal miRNA biomarkers, Creative Biolabs, an expert institution and reliable service supplier, offers optimized exosomal miRNA microarray services to global customers.

Exosomal miRNAs and Microarray

Among identified exosomal RNAs, microRNAs (miRNAs) are those small non-coding RNAs with about 22 nucleotides. These exosomal miRNAs could be transferred between cells with exosome vesicles, thus negatively regulating the biofunctions of recipient cells. The exosomal miRNAs could serve as both suppressors and promoters for tumor cells, and even also have an indirect influence on tumor microenvironment and immune system, emerging as promising biomarkers for minimally invasive diagnosis of tumors.

Fig.1 Mechanism of exosomal miRNA secretion.¹

Currently, exosomal miRNAs are mainly profiled and analyzed by RNA sequencing and microarray technologies. Exosomal miRNA sequencing is directly sequencing the exosomal miRNA-based cDNA library by advanced next-generation sequencers, while, exosomal miRNA microarray captures and detects the target exosomal cDNA sequences through predesigned labeled nucleic acid probes on the chip. In essence, exosomal miRNA sequencing is to directly profile unknown miRNAs, and exosomal miRNA microarray is indirectly profiling unknown miRNAs by known nucleic acid probes.

Fig. 2 Microarray detection of exosome miRNA.²

Customized Exosomal miRNA Microarray Services at Creative Biolabs

Here, our technicians offer optimized exosomal miRNA microarray tests, including probe predesign, exosomal miRNA isolation, and microarray analysis. Of course, exosomal miRNA sequencing services are also our specialty. For exosomal miRNA research, please choose the corresponding service according to your demands and objectives.

1. Consultation and Sample Submission
   • Contact our team about the specifications and objectives of your research.
   • Submit your exosomal miRNA samples according to provided guidelines.
2. Sample Processing
   • Isolate exosomes and extract miRNA using state-of-the-art techniques.
   • Ensure high-quality RNA for downstream microarray analysis.
3. Microarray Experiment Design
   • Custom design your microarray experiment based on your specific targets or pathways of interest.
   • Receive consultation on array platform selection and probe design.
4. Microarray Analysis
   • Perform hybridization and scanning of the microarray slides.
   • Utilize advanced bioinformatics tools for data processing and analysis.
5. Data Interpretation and Reporting
   • Receive comprehensive data analysis reports.
   • Interpretation of results by experienced bioinformaticians.
6. Follow-Up Support
   • Address any additional queries or requests post-analysis.
   • Provide ongoing support for integrating results into your research.

Features

Compared with exosomal miRNA sequencing, exosomal miRNA microarray has features (both advantages and limitations) of:

• Simple to execute and comparatively inexpensive.
• A multitude of samples can be examined concurrently.
• No need for extensive and complex bioinformatic analysis.
• Characterize the miRNA levels based on visualized fluorescence intensity.
• Predesigned probes are required.
• Not applicable to the identification of isoforms and novel genes.

At Creative Biolabs, we have advanced technology platforms, professional scientists, and highly experienced staff to provide you with a full range of standard and tailored exosome services. If you have no idea about the exosomal miRNA service you chose, please do not hesitate to contact us for technical support and a tailored solution.

FAQs

References

1. Guo, Qiao-ru, et al. "The role of exosomal microRNA in cancer drug resistance." Frontiers in oncology 10 (2020): 472. Under Open Access license CC BY 4.0. The image was modified by revising the title.
2. Jia, Hong-Ling, et al. "Sets of serum exosomal microRNAs as candidate diagnostic biomarkers for Kawasaki disease." Scientific reports 7.1 (2017): 44706. Under Open Access license CC BY 4.0, without modification.

• Exosomal Small RNA/miRNA Sequencing
• Exosomal lncRNA Sequencing
• Exosomal circRNA Sequencing
• Exosomal mRNA Sequencing
• Exosomal mRNA Microarray
• Exosomal LncRNA Microarray
• Exosomal ceRNA Microarray
• Exosomal Whole Transcriptome Sequencing