Exosomal Whole Transcriptome Sequencing Service
Overview Services Features FAQs
Creative Biolabs has a complete exosome whole transcriptome sequencing system, which fully combines patented micro nucleic acid library construction technology, NGS technology, and a new biological information sequence comparison algorithm.
Overview
The research object of whole transcriptome sequencing is all RNA that can be transcribed by a specific cell in a certain functional state, mainly mRNA and non-coding RNA such as lncRNA and circRNA. Whole transcriptome sequencing and analysis of long-chain RNA in exosomes can screen the specific biomarkers carried by exosomes and have important significance for the in-depth understanding of the internal mechanisms of diseases or different developmental stages. RNAs such as circRNA and circRNA are fully sequenced to obtain comprehensive exosomal transcriptome information.
Fig. 1 Structure and composition of exosomes.1
Services at Creative Biolabs
Creative Biolabs provides a comprehensive exosome services platform including exosome isolation, characterization, library preparation, NGS sequencing, and data analysis. Customers only need to provide the original samples and we will help you complete the following steps.
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Comprehensive detection type of RNA
Creative Biolabs has complete rRNA removal kits for different species, which can fully retain the type of RNA information.
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Suitable for degradation samples
Creative Biolabs has unique kits suitable for degrading samples, which can retain the RNA information in the degraded samples to a greater extent. Especially suitable for transcriptome detection of clinical samples.
Many gene loci will generate antisense RNA with the opposite transcription direction and important regulatory functions. Conventional library building methods cannot distinguish these antisense RNAs, while Creative Biolabs uses a chain-specific library building method that can accurately detect antisense RNAs, thereby providing more comprehensive transcriptome information.
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Professional biological information analysis
Creative Biolabs has a strong bioinformatics team and is capable of meeting customers' various in-depth data analysis requirements.
Fig. 2 The exosomal transcriptome landscape of breast cancer.2
Features
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Transcript Diversity
Isoform Detection: Captures different isoforms of genes due to alternative splicing events.
Gene Identification: Detects genes that may be related to cancer and other illnesses.
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Gene Expression Quantification
Quantitative Analysis: Provides quantitative data on gene expression across the entire transcriptome.
Differential Expression: Allows gene expression levels to be compared between various treatments or conditions.
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Novel Transcript Discovery
Unannotated Transcripts: Identifies novel or previously unannotated transcripts and splice variants.
Novel Gene Discovery: Helps in discovering new genes that are not present in existing reference genomes.
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Single-Cell Resolution
Single-Cell Sequencing: Can be adapted for single-cell transcriptomics to understand cellular heterogeneity.
Cell Type-Specific Data: Provides insights into gene expression profiles specific to different cell types within a sample.
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Dynamic Range
Wide Detection Range: Captures transcripts with a wide range of expression levels, from low-abundance to highly expressed genes.
High Sensitivity: Offers high sensitivity for detecting low-abundance transcripts.
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Functional Insights
Pathway Analysis: Facilitates understanding of biological pathways and gene networks.
Biomarker Discovery: Identifies potential biomarkers for diseases and therapeutic targets.
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Structural Variation Detection
SNP and Indel Identification: Detects single nucleotide polymorphisms (SNPs) and small insertions/deletions (indels) within the transcriptome.
Alternative Splicing Events: Describes splicing event variations that add to the variety of the transcriptome.
Creative Biolabs provides comprehensive one-stop exosome research services to help accelerate our customers' research. If you are looking for comprehensive exosome transcriptome sequencing results, or you have any puzzles during your exosomal research, please don't hesitate to contact us for professional solutions from the Ph.D. scientist team.
FAQs
Q: What is Full Transcriptome Sequencing for EVs?
A: Full Transcriptome Sequencing for EVs involves sequencing the complete RNA content present within extracellular vesicles. This allows researchers to explore the entire spectrum of RNA molecules, providing insights into the functional cargo of EVs.
Q: How much starting material is required?
A: It is required at least 100 ml cell culture media supernatant per sample provided, or at least 100μL exosomes per sample with ≥0.25 μg/μL concentration. For other sample types, please contact our scientist for advice.
Q: What bioinformatics analyses are included?
A: We offer comprehensive bioinformatics analyses, including differential expression analysis, functional enrichment analysis, and the identification of novel RNA species. Customized analyses can also be discussed with our team.
References
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Xie, Shenmin, Qin Zhang, and Li Jiang. "Current knowledge on exosome biogenesis, cargo-sorting mechanism and therapeutic implications." Membranes 12.5 (2022): 498. Under Open Access license CC BY 4.0. The image was modified by revising the title.
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Liu, Dongdong, et al. "Cross-platform genomic identification and clinical validation of breast cancer diagnostic biomarkers." Aging (Albany NY) 13.3 (2021): 4258. Under Open Access license CC BY 3.0. The image was modified by revising the title.
For Research Use Only. Cannot be used by patients.
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