Custom Oligonucleotide Conjugation Service
Tremendous efforts have been made to improve the oligonucleotide properties by either incorporating chemical modifications in the oligonucleotides structure or covalently linking (conjugation) reporter groups, with biologically relevant properties, to oligonucleotides. Conjugation can be used not only to improve the existing oligonucleotide properties but also to impart entirely new properties. Creative Biolabs provides oligonucleotide conjugation with high quality and yields. Oligonucleotide complexes are produced through our state-of-the-art chemical biology facilities and expertise in cross-linking chemistries.
Oligonucleotide Conjugation
Oligonucleotides are conjugated mostly at 5'- or 3'-termini because of their easy accessibility, however, the 2'-positions of ribose sugar, nucleobases and internucleotide phosphodiester bonds are also used for conjugation. Oligonucleotide conjugation is challenging because subtle changes in structure may influence its biological properties, and therefore, the specific sites for conjugation should be carefully determined. Several synthetic approaches have been developed by Creative Biolabs for facile preparation of oligonucleotide-conjugates, which can be grouped into two major categories:
(i) on-support conjugation, where conjugation is achieved using support-bound oligonucleotides;
(ii) solution-phase conjugation, where oligonucleotides are cleaved from the support, deprotected and purified (if necessary), prior to solution-phase conjugation.
Figure 1. Schematic representation of various strategies used for on-support conjugation synthesis using reported R.
Services
Creative Biolabs offers high-quality oligonucleotide-peptide conjugates by employing selective cross-linking chemistries. We provide a wide variety of synthesis scales, modifications, and purifications to customize your conjugates to exacting requirements. These oligonucleotide-peptide conjugates are used in a variety of biological applications.
Creative Biolabs offers oligonucleotide-antibody conjugation by combining our expertise in the field and over years of a successful track record. Our researchers design new oligonucleotide-antibody conjugates for novel usages on siRNA-antibody delivery in therapeutic applications, vaccines adjuvant or pre-targeting cancer therapeutics.
Creative Biolabs offers oligonucleotide-aptamer conjugates with high quality and yields by using various strategies, according to project specifications. Aptamers are oligonucleotide ligands isolated from combinatorial oligonucleotide libraries by in vitro selection. They fold in solution to form complex three-dimensional structures, which possess a unique capability to selectively bind to the target molecules, ranging from small molecules to complex multimeric structures.
Advantages
- Quality oligonucleotide synthesis
- Flexible bioconjugation chemistries
- Wide choice of target molecules (peptide, antibody, aptamer)
As specialists in bioconjugation, Creative Biolabs employs a variety of cross-linking chemistries including (but not limited to) native ligation chemistry and click chemistry. Each oligonucleotide complex is meticulously monitored during the production cycle, ensuring adherence to our stringent quality control standards. The final product is characterized by mass spectrometry, and its purity is analyzed using high throughput capillary gel electrophoresis. If you are interested in our services, please contact us for more details.
