Tumor Marker Assay

Tumor markers are proteins or other substances found in or released by cancer cells or other body cells during an attack of cancer or benign (noncancerous) conditions. It might tell us something about cancer, like its virulence, which can also open the door to many new ways for scientists to treat tumors. With its in-house scientists and innovative technological platforms, Creative Biolabs is certain to deliver tumor marker assays that enable your project to become a reality.

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Tumor Marker Detection. (Creative Biolabs Authorized)

Why to Detect Tumor Marker?

Tumor markers are essential in immuno-oncology. They allow scientists to assess immune responses and immune escape routes in the tumor microenvironment, thereby defining targets for immunotherapeutic cancer therapies. Cancer markers like PD-L1, CTLA-4, and others involved with immune checkpoints are key to developing immunotherapies that regulate the immune response to cancer. With practical knowledge, tumor marker assays help to push immuno-oncology toward better and more individualized treatments for patients.

Creative Biolabs is the expert in precise tumor marker assays backed by in-house science and state-of-the-art technology. With more than 20 years in the business, we bring industry expertise to the table by offering specific assay solutions for a variety of cancer studies. With our robust platforms and skilled staff, we make sure each assay is scientifically proven and yields reproducible, repeatable results for important cancer research.

Tumor Marker Detection Techniques

Creative Biolabs has an array of tumor markers stratified by cancer, further optimizing the sensitivity and power of your research. This portfolio contains markers for different types of cancer:

Cancer Type	Tumor Markers
Bladder Cancer	EpCAM, FGFR3, VEGF-A, CD47, CD73
Brain Cancer	EGFR, CD44v6, STAT3, MGMT, IDH1
Breast Cancer	HER2, HER3, MUC1, CA15-3, VEGF-A, CD44v6, CEA, ICAM-1
Colorectal Cancer	CEA, CA19-9, GPA33, CD44v6, EpCAM, MMP-9, ICAM-1
Endometrial Cancer	CA125, CEA, VEGF-A, CD44v6
Gastrointestinal Cancers	CEA, CA19-9, GPA33, PSCA, CD44v6, MUC16, ICAM-1, VEGF-A
Head and Neck Cancer	EGFR, CD44v6, VEGF-A, PD-L1, ICAM-1
Hematological Malignancies	CD19, CD20, CD22, CD38, CD138, CD123, FLT3, CCR2, CCR5, CSF1R
Liver Cancer	AFP, GPC3, HGF, VEGF-A, ICAM-1
Lymphoma (B-cell)	CD19, CD20, CD22, CD38, CD138, BAFF, APRIL
Melanoma	NY-ESO-1, STAT3, CD73, TIGIT, LAG-3
Multiple Myeloma	CD38, CD138, BAFF, APRIL, CD56
Non-Small Cell Lung Cancer (NSCLC)	EGFR, NY-ESO-1, VEGF-A, CD73, CD47, PD-L1, TIM-3, LAG-3
Ovarian Cancer	CA125, MUC16, WT1, CEA, VEGF-A
Pancreatic Cancer	CA19-9, CEA, CD73, ICAM-1
Prostate Cancer	PSA, PSMA, PCA3, VEGF-A, CD44v6
Thyroid Cancer	Thyroglobulin, Calcitonin

Tumor Marker Detection Techniques

Creative Biolabs provides an extensive portfolio of tumor marker detection products geared to the needs of the cancer field. Thanks to our advanced tissue, cell, protein, multiplex, and genomic detection systems, we can deliver unparalleled sensitivity and specificity. We use our expertise to design every solution precisely, accurately, and reliably, providing scientists with robust biomarker discovery.

Tissue-Based Detection: IHC, FISH

If you want to detect tumor markers in solid tumor tissues and localize them visually, use IHC or FISH.

Technique	Description	Advantages	Applications
Immunohistochemistry (IHC)	Uses specific antibodies to detect tumor markers in tissue sections.	- Visual and spatial analysis - Widely accessible - Enables localization of marker expression	Commonly used in pathology labs to detect markers like HER2, EGFR, and PD-L1 in solid tumors.
Fluorescence In Situ Hybridization (FISH)	Uses fluorescent probes to detect and localize specific DNA/RNA sequences within tissue samples.	- Visualizes genetic aberrations - Highly sensitive - Useful for solid tumor analysis	Commonly used to detect HER2 amplification in breast cancer and EGFR mutations in lung cancer.

Cell-Based Detection: Flow Cytometry

If you want to detect cell-surface and intracellular markers in single cells, particularly in blood cancers, use Flow Cytometry.

Technique	Description	Advantages	Applications
Flow Cytometry	Detects cell-surface and intracellular markers in single cells, enabling population analysis.	- Fast and multi-parametric - Excellent for rare cell detection - Quantitative	Widely applied in immunophenotyping and the analysis of blood cancers like lymphoma and leukemia.

Protein-Based Detection: ELISA, SMC, Mass Spectrometry

If you want to detect protein biomarkers in serum, plasma, or tissue extracts with high sensitivity and specificity, use ELISA, SMC, or Mass Spectrometry.

Technique	Description	Advantages	Applications
Enzyme-Linked Immunosorbent Assay (ELISA)	A widely used assay that utilizes antibodies to detect and quantify specific tumor markers.	- High sensitivity and specificity - Cost-effective - Rapid results	Ideal for high-throughput screening and monitoring known tumor markers like CA15-3, PSA, and CEA.
Single Molecule Counting (SMC)	Detects individual protein molecules for ultra-sensitive quantification.	- High sensitivity - Minimal sample volume needed - Detects low-abundance markers	Ideal for monitoring markers in plasma, serum, and other low-concentration samples.
Mass Spectrometry (MS)	Analyzes protein and peptide composition to identify unique tumor markers.	- High specificity - Quantitative results - Suitable for proteomic studies	Applied in identifying and validating protein biomarkers in solid and liquid samples.

Multiplex Detection: Multiplex Assay (MILLIPLEX), Luminex xMAP Technology

If you want to detect multiple biomarkers simultaneously in a single sample, saving time and resources, use Multiplex Assays (e.g., MILLIPLEX) or Luminex xMAP Technology.

Technique	Description	Advantages	Applications
Multiplex Assay (e.g., MILLIPLEX)	Allows simultaneous detection of multiple tumor markers within a single sample.	- Saves time and reduces sample volume - High data density - Excellent for studying complex biomarker interactions	Suitable for comprehensive profiling of multiple markers in cancer research.
Luminex xMAP Technology	Utilizes color-coded beads for multiplex biomarker detection in a single reaction.	- High throughput - Multi-marker detection - Precise and efficient	Suitable for biomarker discovery and validation studies in large sample sets.

Genomic Detection: NGS, ddPCR

If you want to detect DNA/RNA mutations, methylation patterns, and other genomic markers, especially for liquid biopsies and genetic profiling, use NGS or ddPCR.

Technique	Description	Advantages	Applications
Next-Generation Sequencing (NGS)	An advanced technique that sequences DNA/RNA to identify genetic mutations and methylation markers.	- High-resolution detection - Enables analysis of cfDNA and ctDNA - Capable of detecting rare mutations	Frequently used for liquid biopsies and early-stage cancer detection.
Digital Droplet PCR (ddPCR)	A digital PCR technique that quantifies low-abundance targets with high precision.	- Ultra-sensitive for detecting low-level DNA/RNA - Suitable for cfDNA and ctDNA - Accurate quantification	Ideal for detecting mutations and methylation in liquid biopsy samples for cancer diagnostics.

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Great Partners of Creative Biolabs. (Creative Biolabs Authorized)

Effective Immunohistochemistry (IHC) Service

"Creative Biolabs' IHC service was instrumental in our research on HER2 expression in breast cancer. The staining process was clean and well-executed, and we were able to visualize the localization of tumor markers within tissue samples with ease."

Highly Reliable Assays for Cancer Research

"As a research lab focused on immuno-oncology, we needed highly reliable tumor marker assays. Creative Biolabs delivered exactly what we needed. Their customized assays for breast cancer markers were precise and provided clear, reproducible results. We are confident in their technology and look forward to future collaborations."

Precise Flow Cytometry Service

"We chose Creative Biolabs for their flow cytometry service to analyze blood cancer markers, and they exceeded our expectations. Their system was fast, and the results were accurate, providing us with the multi-parametric data needed to analyze rare cell populations effectively."

Outstanding ELISA Assay for Protein Markers

"We used Creative Biolabs' ELISA assays to monitor key protein markers in our cancer samples. The assays were sensitive and cost-effective, providing reliable quantitative data that allowed us to track tumor progression with confidence."

Effective Immunohistochemistry (IHC) Service

"Creative Biolabs' IHC service was instrumental in our research on HER2 expression in breast cancer. The staining process was clean and well-executed, and we were able to visualize the localization of tumor markers within tissue samples with ease."

Highly Reliable Assays for Cancer Research

"As a research lab focused on immuno-oncology, we needed highly reliable tumor marker assays. Creative Biolabs delivered exactly what we needed. Their customized assays for breast cancer markers were precise and provided clear, reproducible results. We are confident in their technology and look forward to future collaborations."

Why Choose Us?

High Sensitivity and Specificity

Creative Biolabs offers cutting-edge techniques such as SMC and ddPCR, providing ultra-sensitive detection for low-abundance biomarkers, essential for early-stage cancer detection and liquid biopsies.

Advanced Genomic and Proteomic Profiling

We provide high-resolution information on genetic and protein-level biomarkers via NGS and Mass Spectrometry. These approaches enable complete description of the biology of tumors, which helps to create targeted therapies and precision medicine.

Single-Cell Analysis

Techniques like Flow Cytometry and FISH provide insights at the single-cell level, critical for analyzing heterogeneous tumors and identifying specific cell populations within a sample, enhancing the accuracy of diagnostics in complex cancers.

Customized Solutions

Our advanced technology platform allows you to develop custom assays according to your research requirements and with adaptability of the sample type (e.g., blood, tissue, urine) and the number of assays (e.g., high-throughput ELISA, custom multiplex assays).

At Creative Biolabs, we collaborate with our customers to define the specific needs and requirements to deliver the partnership goals. Creative Biolabs wants to be the solution provider you always turn to. With our advanced technologies and well-experienced staff, we are dedicated to accelerating your program and shortening your overall development time. For more information, feel free to contact us.

FAQs

What tumor markers do you provide for different types of cancers?

We provide a comprehensive suite of tumor markers for various cancers: breast, lung, thyroid, prostate, colorectal, and haematological cancers. You can select any number of genetic, protein, and immune checkpoint markers to best suit your research needs.

How do your multiplex assays work and what are their benefits?

Our multiplex assays enable us to capture multiple tumor markers in a single sample. This technique minimizes sample use and processing, enabling ultra-high throughput for biomarker profiling, which can be used for large-scale cancer research.

Can you offer customized tumor marker assays for specialized research purposes?

And yes, we offer completely customizable tumor marker tests. Whether your work concerns a cancer type or special markers, we can customize our assays to your project needs and give you the most relevant and accurate data possible.

How sensitive are your ELISA assays to detect tumor markers?

Our ELISAs are highly sensitive and allow us to identify low-density tumor markers in blood, plasma, or tissue. We test every assay to ensure sensitivity and specificity and give you exact quantitative results.

Why is your NGS service ideal for liquid biopsies?

Our NGS service analyses DNA/RNA from liquid biopsy samples with high resolution, which allows us to detect rare mutations and genetic changes. It is particularly suited for early-stage cancer diagnosis and genetic analysis and offers practical information for personalized treatment.

What tumor markers are suitable for detecting immune escape mechanisms in tumors?

PD-L1, CTLA-4, and other immune checkpoint inhibitors serve as critical indicators of immune escape mechanisms. These markers are central to the science of immuno-oncology and are important targets for creating effective immunotherapies.

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