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ZO1 Expression Analysis

Creative Biolabs is pleased to offer a ZO1 expression analysis service as part of our iPSC-facilitated therapy development for ABD. This service aims to investigate the potential role of ZO1 expression in the development and progression of AMD using iPSCs.

Significance

ZO1 (zonula occludens 1) is a tight junction protein critical for maintaining the integrity and function of RPE cells, which plays a crucial role in the pathogenesis of AMD. By analyzing ZO1 expression levels and localization in iPSC-derived RPE cells, researchers can:

  • Understand the role of ZO1 in RPE function and AMD progression.
  • Determine possible treatment targets and avenues for AMD.
  • Evaluate the efficacy and safety of AMD therapy development.

Service Process

  1. Consultation and project design. We first conduct a detailed consultation to determine the unique needs of each customer.
  2. iPSC differentiation and retinal cell generation. We ensure efficient and reliable cell differentiation, producing high-quality retinal cells for further analysis.
  3. Samples collection and staining with specific antibodies against ZO1. We meticulously process samples to prepare them for analysis. The samples are then stained with specific antibodies targeting ZO1.
  4. ZO1 expression analysis using advanced imaging techniques. We conduct a detailed analysis of ZO1 expression in the stained samples. This makes it possible for us to observe and measure the distribution and intensity of ZO1 expression.
  5. Quantitative analysis of ZO1 expression levels and localization patterns. Our team performs a rigorous quantitative analysis to assess the levels of ZO1 expression and its localization patterns within the cells.
  6. Generation of a comprehensive report with detailed findings and recommendations.

Fig.1 ZO1 expression was detected by immunochemical staining. (Caruso, et al., 2023)Fig.1 Immunocytochemistry evaluation of ZO-1 staining in resting RPE cells and RPE cells exposed to treatments.1

Applications

  • Characterization of ZO1 expression in iPSC-derived retinal cells.
  • Comparison of ZO1 expression between healthy and diseased iPSC-derived retinal cells.
  • Evaluation of the effects of potential therapeutic compounds on ZO1 expression levels.

Deliverables

  • Raw data from ZO1 expression analysis.
  • Comprehensive report with detailed experimental procedures, results, and implications for AMD research.
  • Expert consultation for further data interpretation and recommendations for future research.

FAQs

1. How long does the ZO1 expression analysis service typically take?

The timeline for the service may vary depending on the particular needs of the project. Our staff will collaborate closely with you to establish a timeline that meets your research needs.

2. Can you provide customized analysis beyond ZO1 expression?

Yes, our team of experts can offer additional analyses tailored to your specific research goals. Please discuss your requirements with our project manager during the consultation phase.

At Creative Biolabs, we offer a ZO1 expression analysis service to support the advancement of iPSC-facilitated therapy discovery for AMD. For more information about our ZO1 expression analysis service or to discuss your specific research needs, please contact us.

Reference

  1. Caruso, Giuseppe, et al. "Carnosine Counteracts the Molecular Alterations Aβ Oligomers-Induced in Human Retinal Pigment Epithelial Cells." Molecules 28.8 (2023): 3324.

For Research Use Only. Not For Clinical Use.