Carrot-derived Exosome Research and Application

Studies have found that carrot-derived exosomes exhibit the ability to mitigate cellular damage caused by oxidative stress, with the potential for the treatment of myocardial infarction and Parkinson's Disease. Creative Biolabs provides high-quality services to assist in the study of carrot-derived exosome production and functional development, contributing to the exploration of carrot-derived exosomes as drug candidates.

Features of Carrot-derived Exosomes

Carrots as exosome donors are easy to culture and take.
Carrots are rich in nutrients, especially recognized carotenoids, as well as high levels of vitamins, minerals such as calcium and iron, and fiber.
Carrot-derived exosomes have the potential to package active nutrients from carrots during formation.
The high yield and high purity of carrot-derived exosomes compared to other plant sources have also been found in studies.
Carrot-derived exosomes are known to carry excellent antioxidant activity.

Isolation of Carrot-derived Exosomes

Wash carrots in distilled water to remove surface dirt and pesticides.
Extraction of carrot juice by blender.
Centrifugation at low and high speeds to remove large carrot fragments.
Concentration of carrot juice by ultrafiltration.
Separation of carrot-derived exosomes from carrot juice by size exclusion chromatography.
Elution of carrot exosomes from size exclusion chromatography with PBS.

Research on Carrot-derived Exosomes

Research	Conclusion
Characterization of carrot-derived exosomes.	The results show that their size is about 140 nm, with negative electronegativity, sharing the recognized exosome characteristics.
Effect of carrot-derived exosomes on cardiomyocytes.	Carrot-derived exosomes did not cause damage to mouse-derived cardiomyocytes. Tracer assay of fluorescent dye-labeled exosomes demonstrated that carrot-derived exosomes can be transferred to target cells for their active potency.
Antioxidant effects of carrot-derived exosomes in cardiomyocytes.	Carrot-derived exosomes were able to down-regulate abnormally high levels of ROS in H₂O₂-induced cardiomyocytes, dose-dependently rescuing apoptosis and thus inhibiting the reduction of cell viability due to oxidative stress.
Antioxidant mechanisms of carrot exosomes in cardiomyocytes.	RT-PCR and protein blotting analyses demonstrated that the antioxidant potency of carrot exosomes in cardiomyocytes was achieved by inhibiting the reduction in the expression of antioxidant proteins (HO-1 and NQO-1) and their transcription factor (Nrf-2).
Effects of carrot-derived exosomes on neuroblastoma cells.	Similar to the effects on cardiomyocytes, treatment with carrot-derived exosomes in a neurotoxin-induced neuroblastoma model also resulted in SH-SY5Y cells being resistant to oxidative stress, indicating the potential development of carrot-derived exosomes as drug candidates against Parkinson's disease.

Fig. 1 Carrot-derived exosomes exert antioxidant effects and their molecular mechanisms.¹

As carrots are known to exhibit a variety of healthful components including carotenoids and vitamins, carrot-derived exosomes have been viewed as promising drugs that serve a broad range of roles including, but not limited to, antioxidant activity and mitigation of cellular damage. Creative Biolabs is committed to the advancement of vegetable exosomes, including carrot-derived exosomes, and can provide customized solutions. Please contact us with your research requirements for carrot-derived exosomes.

Reference

Kim, Do Kyung, and Won Jong Rhee. "Antioxidative effects of carrot-derived nanovesicles in cardiomyoblast and neuroblastoma cells." Pharmaceutics 13.8 (2021): 1203.

Plant Exosome Isolation