Shiitake Mushroom-derived Exosome Research and Application

By conducting an in-depth investigation into the components and functions of exosomes derived from Shiitake mushrooms, we can provide a scientific foundation for the development of new foods, drugs, or health products to better meet people's needs for health and nutrition. Creative Biolabs offers state-of-the-art technical support for the development of exosomes derived from Shiitake mushrooms.

The Magical Powers of Shiitake mushrooms

Shiitake mushrooms are a common edible fungus with various benefits and medicinal value:

Immune Modulation: Shiitake mushrooms contain polysaccharides that help enhance the immune system's function, improving resistance to infections and diseases.
Blood Pressure and Cholesterol Regulation: The polysaccharides in Shiitake mushrooms can help regulate blood sugar levels, lower blood pressure, and reduce blood lipid levels, contributing to cardiovascular health.
Antioxidant Properties: Shiitake mushrooms are abundant in polyphenols with strong antioxidant effects, helping neutralize free radicals, slow down cellular aging, and maintain overall health.
Anti-Cancer Effects: Shiitake mushrooms exhibit inhibitory effects on cancer cells, reducing the risk of cancer and being considered a natural anti-cancer ingredient.
Digestive Aid: Shiitake mushrooms are rich in dietary fiber, promoting intestinal motility and digestion, preventing constipation, and gastrointestinal issues.
Cardiovascular Protection: Polyphenols and plant fibers in Shiitake mushrooms aid in clearing deposits in the blood, lowering the risk of cardiovascular diseases.

Research on Shiitake Mushroom-derived Exosomes

Due to the abundance of beneficial bioactive components in Shiitake mushrooms and the fact that exosomes are vesicles secreted by cells, typically carrying cell signaling molecules such as proteins, nucleic acids, and lipids crucial for intercellular communication and signaling, studying exosomes derived from Shiitake mushrooms can explore whether they carry beneficial components and if these components have therapeutic potential.

Title: Protective role of shiitake mushroom-derived exosome-like nanoparticles in d-galactosamine and lipopolysaccharide-induced acute liver injury in mice.

Research Objectives: To investigate whether exosomes derived from Shiitake mushrooms have the potential to inhibit NLRP3 inflammasome activity and alleviate acute liver injury.

Research Methods:

Successful extraction of exosomes from seven common edible mushrooms and analysis of their morphology, size, and composition.
In vitro validation of anti-inflammatory functions: In cell-based experiments, the inhibitory effects of exosomes from different edible mushrooms on NLRP3 inflammasome activity in macrophages, including their impact on cytokines like IL-1β, IL-18, IL-6, and downstream events such as cell death and Casp1 cleavage.
Acute liver injury model: In a mouse model of acute liver injury, administration of exosomes derived from Shiitake mushrooms followed by induction of acute liver injury using GalN/LPS.
Assessment at physiological and molecular levels: Evaluation of liver histology, serum markers (ALT, AST), and cytokine levels (IL-1β, IL-18, IL-6, etc.) to study the protective effects of shiitake mushroom-derived exosomes against acute liver injury.
Analysis at the gene and protein levels: Investigation of the impact of shiitake mushroom-derived exosomes on the expression of inflammation-related genes (Nlrp3, Il1b, Il6, Tnf) and proteins (Pro-IL-1β, NLRP3).

Research Findings:

Anti-inflammatory Function: Only exosomes derived from Shiitake mushrooms demonstrated a powerful ability to inhibit NLRP3 inflammasome activity, including the suppression of IL-1β, IL-18, IL-6 release, and a reduction in cell death.
Protection against Acute Liver Injury: In a mouse model of acute liver injury, administration of exosomes from Shiitake mushrooms significantly improved liver health and reduced the severity of liver damage.
Molecular Mechanisms: Shiitake mushroom-derived exosomes exerted their anti-inflammatory effects by downregulating inflammation-related genes (Nlrp3, Il1b, Il6, Tnf) and proteins (Pro-IL-1β, NLRP3).

Conclusion: Shiitake mushroom-derived exosomes exhibit significant anti-inflammatory properties, especially in the inhibition of NLRP3 inflammasome activity. These exosomes also show potential in protecting the liver from injury in a mouse model of acute liver damage, suggesting that they could be a potential candidate for the treatment of acute liver injury and other inflammatory diseases.

Undoubtedly, exosomes derived from Shiitake mushrooms are likely to become a highlight in future research, attracting the attention of more researchers. Right now, Creative Biolabs offers a wide range of services for extracting and developing plant-derived exosomes. If you have any exosome-related needs or questions, feel free to contact us at any time.

Plant-Derived Exosome Isolation

Plant-Derived Exosome Identification

High-Throughput Screening Analysis (Proteins, RNA, Lipids and Metabolites)

Target Analysis

In Vitro Functional Discovery of Plant-Derived Exosomes

In Vivo Functional Discovery of Plant-Derived Exosomes

Large-Scale Production of Plant-Derived Exosomes

Reference

Liu, B.; Lu, Y.; et al. Protective role of shiitake mushroom-derived exosome-like nanoparticles in d-galactosamine and lipopolysaccharide-induced acute liver injury in mice. Nutrients. 2020. 13;12(2)

Plant Exosome Isolation