Phosphorylation

Oligonucleotides (Oligos) can be modified in several ways by utilizing the active groups of the nucleotide or creating nucleotide analogues. One important modification is phosphorylation that means the chemical addition of a phosphoryl group (PO^3-) to an organic molecule. It is important in the fields of biochemistry and molecular biology because it's a key reaction in protein and enzyme function, sugar metabolism, and energy storage and release. Powered with advanced technologies and experienced staff in oligos modifications, Creative Biolabs provides high-quality oligos synthesis services with phosphorylation modification. We aspire to become the trusted service provider of your first choice.

An Overview of Phosphorylation

Phosphorylation is the most common and important molecular mechanism of acute and reversible regulation of protein function. Through protein phosphorylation, protein function is regulated in response to extracellular stimuli both inside and outside the cell. To date, protein phosphorylation has become a central focus within drug discovery because of the result of the identification and validation of promising therapeutic targets such as protein kinases, protein phosphatases, and phosphoprotein binding domains. Likewise, oligos phosphorylation plays an important role in oligos modifications and helps researchers to study DNA and RNA.

Types of Phosphorylation Modification

At Creative Biolabs, we can provide two ways of phosphorylation modification, in which phosphates can be added at 5' end or 3' end of an oligo. 5' phosphorylation is needed if an oligo is used as a substrate for DNA ligase. 3' phosphorylation will inhibit degradation by some 3'-exonucleases and can be used to block extension by DNA polymerases.

• 5' phosphorylation
  

  Oligonucleotides require a 5' phosphate for ligation. This phosphate can be added enzymatically using polynucleotide kinase, or can be added chemically during the oligo synthesis. Oligos containing a 5'-phosphate group have been used in molecular biology for a variety of purposes: e.g., as linkers and adapters, in cloning and gene construction, and in the ligase chain reaction.

• 3' phosphorylation
  

  Phosphorylated oligos are useful in changing the susceptibility of a sequence to hydrolysis by an exonuclease, that is the reason why Fluorescence Resonance Energy Transfer (FRET) probes labeled with an acceptor fluorophore require a 3' phosphate.

Why Creative Biolabs?

Creative Biolabs offers high-quality products aided by our advanced technologies and researchers. All operations are under stringent manufacturing, and QC & QA standards. Moreover, we provide various methods of purification, specifications and delivery forms, and flexible synthesis scales are available based on customers' requirements. At the same time, we offer one-stop services from the design to purification and delivery to customers.

Creative Biolabs' advanced technologies and highly experienced staff are committed to offering oligos synthesis with phosphorylation modification to improve your program and decrease the overall development timeline. Creative Biolabs will discuss with our partner customers to define their specific requirements and expectations to meet the partnership requirements. Whatever your requirements are, we can adopt suitable technologies, diverse therapeutic expertise and make researches shorter and more efficient. If you have any special requirements in phosphorylation modification, please don't hesitate to contact us.