Creative Biolabs has established an advanced platform to develop synthetic gene circuits for cancer immunotherapy- turning cancer cells against themselves. We have developed several efficient strategies to engineer the human pancreatic cancer cell line PSN-1 to turn pancreatic cancer cells against themselves. Our comprehensive portfolio provides reliable support to help our clients' related projects.

Properties of PSN-1

PSN-1 was derived from pancreatic adenocarcinoma tissue. After transplantation into nude mice the PSN-1 cell line was established from the xenograft. PSN1 cells are unique with amplification of both c-myc and activated c-Ki-ras with a point mutation. Studies have shown that the amounts of cmyc and c-Ki-ras transcripts are increased markedly in PSN-1 cells.

Fig.1 Phase microscopic appearance of PSN-1 cells. (Yamada, et al.,1986)

Pancreas Cancer Cell Line PSN-1 Engineering Services at Creative Biolabs

To inspire the lymphangiogenic potentiation of immunotherapy to turn cancer cells against themselves, Creative Biolabs has developed innovative platforms. Based on these advanced platforms, a comprehensive range of Cancer Cells are available to be engineered. PSN-1 is a potential cell line that can be used in the research of the treatment of pancreatic cancer. Our scientists are proficient in multiple Genetic Methods for PSN-1 engineering, including Retrovirus, Lentivirus, Adenovirus, Adeno-associated Virus, and CRISPR-based Methods. Our strategies for engineering cancer cells for self-destruction, include but are not limited to:

• Engineering Cancer Cells with Cytokine Overexpression for Stronger T Cell Activation
• Engineering Cancer Cells with Target-specific Aptamer for Cancer Immunotherapy
• Engineering Cancer Cells With Aptamer-siRNA for Enhanced Antitumor T Cell Immunity
• Engineering Cancer Cells with Synthetic Immunomodulatory Gene Circuits for Cancer Immunotherapy
• Engineering Cancer Cells with Targeted siRNA For Cancer Immunotherapy

What's more, Ex Vivo Immune Reactivity and Toxicity are needed to assess the effect of self-destruction cancer cells. Our methods for ex vivo immune reactivity assessment include Cytokine Production Measurement and Chemokine Production Measurement. Our methods for Toxicity assessment of self-destruction cancer cells include but are not limited to:

• Quantification of Alanine Aminotransferase (ALT)
• Quantification of Alkaline Phosphate (ALP)
• Quantification of Amylase
• Quantification of Calcium
• Quantification of Glucose
• Quantification of Cholesterol
• Quantification of Electrolyte
• Quantification of Creatinine Kinase
• Quantification of Total Bilirubin
• Quantification of Total Protein

Scientists at Creative Biolabs lend their expertise and assist you in developing a strategy that is right for your program and help you overcome any hurdles that you may face during the R&D phase. For more detailed information, please feel free to contact us or directly send us an inquiry.

Reference

1. Yamada, H.; et al. Establishment of a human pancreatic adenocarcinoma cell line (PSN-1) with amplifications of both c-myc and activated c-Ki-ras by a point mutation. Biochemical and biophysical research communications. 1986, 140(1): 167-73.

For Research Use Only | Not For Clinical Use