Internalizing Modification Services

With more than a decade of experience in antibody engineering and chemical/biochemical modifications, Creative Biolabs is dedicated to applying and improving innovative new technologies and methodologies to promote the field of next-generation antibody development.

As an emerging field, the development of antibodies against cytoplasmic targets is gaining more attention. Many associated techniques have been developed to facilitate those projects. Antibodies or fragmented antibodies (e.g. scFv and single domain antibody V_HH) can be raised against a cytoplasmic target by conventional methods such as hybridoma, phage display antibody library screening, and plasma cell sorting. However, different from antibodies against cell surface antigens, the evaluation of their in vitro efficacy requires their entering into the cytosol to reach the intracellular targets. Based on the assumption that smaller size increases the chance of membrane penetration, many anti-cytoplasmic protein antibodies are developed as antibody fragments. However, sometimes the small size does not present enough penetration and further modifications are still required to enhance the internalization capacity. As a leading service provider in antibody engineering and modification, Creative Biolabs has adopted several methods to promote the development of anti-cytoplasmic transbodies.

Cell-Penetrating Peptides (CPPs)

CPPs are small peptides discovered in the late 1980s that contain unique sequence features. These sequence features render those peptides either highly charged or amphipathic. Proteins bearing CPPs often show higher cytosol entering potential, despite their source of origin. Creative Biolabs offers CPP-related modification services to promote the internalization capacity of either antibodies or proteins.

Lectin-Mediated Endocytosis

Some cell surface receptors bear lectin modules that recognize certain glycan formation and tend to form lectin-glycan complexes that will ultimately be internalized. We refer to this process as lectin-mediated endocytosis. With our advanced glycol-engineering platform, scientists at Creative Biolabs can help create antibodies bearing glycans via glycol-engineering and chemo-enzymatic glycan modification to take full advantage of the lectin-mediated endocytosis process.

Creative Biolabs is dedicated to providing high-quality services to facilitate your project. If you are interested in our transbody-related services, please feel free to contact us for more details.

Reference

1. Trabulo, S.; et al. Cell-penetrating peptides—mechanisms of cellular uptake and generation of delivery systems. Pharmaceuticals. 2010, 3: 961-993.

Platform:

Intracellular Antibody Development Platform

All of our antibody products and services can only be used for preclinical research studies. Do not use them on humans.