Perilipin Expression Assay

Creative Biolabs launches perilipin expression assay based on iPSC-derived human sebocytes, which can help you study the effects of drug candidates on lipid metabolism and sebocyte function, accelerating your progress in screening and characterizing acne and hyper/hyposeborrhea drugs.

Perilipin Expression Assay Based on iPSC-Derived Sebocyte Model

Perilipin, also referred to as lipid droplet-associated protein or PLIN, plays a crucial role in lipid metabolism and storage. It is primarily found in adipocytes and sebocytes, where it regulates lipolysis, the breakdown of stored lipids. Perilipin plays a role in sebogenesis and sebum lipid accumulation in vitro, as well as sebaceous gland growth in vivo. Perilipin expression is tightly regulated and can be influenced by various factors such as hormonal signals, nutritional status, and metabolic conditions. Increased perilipin expression is associated with enhanced lipid storage, while decreased expression can promote lipid mobilization and utilization.

Fig.1 Hormonal regulation of PLIN1-dependent lipolysis in adipocytes.¹

In recent years, the advent of iPSC technology has revolutionized the field of dermatological research by enabling the generation of human sebocytes from iPSCs. The perilipin expression assay utilizing iPSC-derived human sebocytes serves as a valuable tool for investigating the impact of drug candidates on lipid metabolism and sebocyte function. The following is the key workflow.

1. Cell Culture: iPSCs are first differentiated into sebocytes. Maintain cells in appropriate culture conditions to ensure their health and functionality.
2. Treatment with Drug Candidates: The iPSC-derived sebocytes are treated with different drug candidates or new compounds that are being evaluated for their potential in acne treatment.
3. Determination of Perilipin Expression: Immunofluorescence, quantitative PCR, and Western blot can be used to detect the expression level of perilipin in cortical cells.
4. Analysis and Interpretation: The results are analyzed to determine how the drug candidates impact perilipin expression in the sebocytes.

Perilipin Expression Assay for Acne and Hyper/Hyposeborrhea

Acne vulgaris is a prevalent skin condition characterized by sebum overproduction, inflammation, and the proliferation of Propionibacterium acnes. Sebaceous glands, which produce and secrete sebum, play a significant role in the pathogenesis of acne. Lipid metabolism in these glands is regulated by perilipin. Through perilipin expression assay based on iPSC-derived human sebocytes, researchers can assess their potential effects on sebaceous gland function and sebum production. This assay provides crucial insights into the mechanisms underlying acne pathogenesis and imbalances in sebum secretion, offering a platform for screening novel compounds aimed at addressing hyper or hyposeborrhea-related skin conditions.

Creative Biolabs is dedicated to offering full services for stem cell treatment research. You can get a variety of cells derived from iPSC and a variety of analysis services for regulating diseases here. Please contact us for more details.

Reference

1. Itabe, Hiroyuki, et al. "Perilipins: a diversity of intracellular lipid droplet proteins." Lipids in health and disease 16 (2017): 1-11.

For Research Use Only. Not For Clinical Use.