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Creative Biolabs provides tailored, high-caliber functional assays for complement C1r, utilizing advanced technologies and vast scientific expertise to aid clients in advancing therapies targeting the complement system.
Overview of Complement C1r
C1 serves as the initial component in the classical complement cascade, consisting of three distinct parts: one copy of C1q, the pattern recognition molecule, and two copies each of C1r and C1s, which are serine proteases interconnected via a noncovalent, Ca2+ dependent complex. The proteolytic subunits, C1r and C1s, form a Ca2+-dependent linear tetramer with the sequence C1s–C1r–C1r–C1s. Within the C1 assembly, the C1r/C1s tetramer adopts a figure-eight configuration positioned between the globular domains. Ionic interactions between a singular basic residue on the six C1q stalks and acidic residues on C1r/C1s secure the tetramer. A C1r deficiency is associated with the onset of systemic lupus erythematosus (SLE).
Fig.1 Network of complement proteins and proteases.1
Complement C1r Functional Assay Introduction
Method
The complement C1r functional assay is a laboratory method designed to assess the activity and function of the C1r component within the complement system across various samples, utilizing ELISA techniques. Furthermore, hemolytic tests are available to detect C1r, which in serum is present as an inactive proenzyme and is converted into an active enzyme when the C1 complex is triggered. Activation takes place when C1 engages with antibody-antigen complexes, positioning C1r as the initial protease to initiate the cascade. A functional pathway can be reconstituted by introducing purified C1r enzyme or proenzyme to C1r-depleted serum, confirming the presence and functionality of other essential complement components.
Analysis
A statistical evaluation of C1r ELISA data was performed. For comparisons between two groups, either a 2-tailed t-test (parametric) or a paired t-test (non-parametric) was employed, whereas comparisons across multiple groups were conducted using a 1-way ANOVA followed by a post hoc multiple comparison test.
Workflow of Complement C1r Functional Test
Applications
Utilizing for Disease Detection: Gain-of-function variants in C1r may increase the risk of bacterial infections, potentially playing a role in periodontal Ehlers-Danlos syndrome. Given that C1r is activated across various pathological states and associated with inflammation, autoimmunity, and cancer progression, it is becoming a significant biomarker for the diagnosis and management of diverse diseases.
Drug development: Antibody binding to C1q hinders the activation of associated serine proteases (C1s–C1r–C1r–C1s), thus blocking further complement activation. Dysregulated activation of the classical pathway is linked to antibody-mediated inflammatory disease processes. Exploring the potential of anti-C1r antibodies in pre-clinical research could underscore their promise as therapeutics in managing classical pathway disorders driven by antibodies.
Related Products
Featured Services
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Total Complement Activity Test
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Complement Multiplex Assay Services
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Creative Biolabs offers total complement activity test services for our clients. Total complement activity tests include CH50, AH50, and LP50.
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Creative Biolabs specializes in sophisticated complement multiplex assay services to facilitate complement protein characterization and therapy development, enabling simultaneous analysis of multiple complement proteins.
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Learn more
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Learn more
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Protocols
Questions & Answer
A: Creative Biolabs specializes in supporting drug development by providing highly customized complement C1r functional assays. Our services aid researchers in exploring the potential of anti-C1r antibodies, which are promising therapeutics for diseases driven by the classical complement pathway. If you're working on innovative therapies, we’d be thrilled to collaborate with you.
A: A complement C1r functional assay is a cutting-edge test that evaluates the activity of the C1r protein, a key player in the classical complement pathway of the immune system. At Creative Biolabs, we tailor this assay using advanced ELISA techniques and hemolytic tests to meet your research or therapeutic needs. This assay is crucial for studying autoimmune diseases, inflammatory conditions, and even cancer progression.
A: Creative Biolabs utilizes cutting-edge technologies and stringent quality control protocols to guarantee the highest level of accuracy in our complement C1r assays. From ELISA methods to statistical evaluations using t-tests and ANOVA, we leave no stone unturned in delivering reliable and reproducible results. Our scientific expertise ensures your research is in good hands.
A: We provide tailored complement C1r assays that can be applied to disease detection, including conditions. By evaluating C1r activity, we help uncover its role in pathological states, providing vital insights for diagnostics and management. Let us support your research and diagnostics needs.
Resources
Creative Biolabs is committed to delivering efficient and practical services centered on the complement system, supported by a reliable and standardized assay platform. We offer tailored C1r functional assays designed for rapid assessment of complement activity. We welcome you to contact us with any inquiries or interests related to the complement function assay.
Reference
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Lu, Jinhua, and Uday Kishore. "C1 complex: an adaptable proteolytic module for complement and non-complement functions." Frontiers in Immunology 8 (2017): 592. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
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