Complement C3b Functional Test

Creative Biolabs provides bespoke, high-caliber functional assays for complement C3b, utilizing cutting-edge technologies and deep scientific knowledge to support clients in advancing therapies targeting the complement system.

Overview of Complement C3b

C3b, the larger fragment resulting from the proteolytic cleavage of complement component 3, is regarded as a crucial element of the innate immune system. It exhibits potent opsonizing activity, labeling pathogens, antigen-antibody complexes, and apoptotic cells for phagocytic clearance. Furthermore, C3b contributes to the assembly of a C3 convertase upon association with Factor B, and a C5 convertase when it combines with C4b and C2b or when another C3b fragment associates with the C3bBb complex.

Fig.1 The convergence of three distinct extracellular complement activation pathways results in a shared terminal pathway.¹

Complement C3b Functional Assay Introduction

Method

The complement C3b functional assay is a laboratory technique designed to evaluate the activity and functionality of the C3b component within the complement system across various samples. This assessment is achievable through the use of hemolytic tests and solid-phase sandwich ELISA methods. We also provide a C3b Deposition assay which measures the amount of complement-mediated C3b deposited on cell surfaces after activation of the alternative pathway (AP).

Analysis

C3b, a pivotal component of the alternative pathway C3 convertase (C3bBb), interacts with various regulators and receptors, thereby influencing immune system function. Rigorous evaluation and quantification of C3b's role can be achieved through functional or activity assays. Statistical analysis of ELISA data was conducted. Comparisons between two groups utilized either a 2-tailed t-test (parametric) or paired t-test (non-parametric), while comparisons among multiple groups were performed using a 1-way ANOVA followed by multiple comparison tests.

Workflow of Complement C3b Functional Test

Applications

• Disease Detection: Complement protein C3b is associated with several conditions, including C3 glomerulopathy and immune complex glomerulonephritis. Assessing C3b activity facilitates not only the diagnosis of these disorders but also the tracking of disease progression.
• Acting as a biomarker: C3b, a fragment generated during the activation of complement protein C3, has been reported in certain studies to exhibit a positive correlation with the severity of diseases. This potential makes C3b an attractive biomarker for predicting diseases such as periodontitis and exploring their pathogenesis.
• Drug Development: The functional assay for C3b offers novel opportunities for tailoring anti-complement therapies to improve disease treatment.

Related Products

Featured Services

Total Complement Activity Test	Complement Function/Activity Test
Creative Biolabs offers total complement activity test services for our clients. Total complement activity tests include CH50, AH50, and LP50.	Creative Biolabs provides complement function or activity tests designed to identify patients with complement deficiencies which are also increasingly utilized in developing complement-targeted therapeutics.
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Questions & Answer

Resources

Complement Receptor of the Immunoglobulin Superfamily/CRIg

Alternative Pathway

Complement Regulator of Complement System: RCA/CCP Family

Creative Biolabs is dedicated to providing effective and practical complement-related services, founded on a dependable and standardized assay platform. We offer tailored C3b functional assays designed for the rapid assessment of complement activity. We encourage you to reach out to us to contact us to discuss any interests or inquiries you may have concerning the complement function assay.

Reference

1. Girardi, Guillermina, et al. "Essential role of complement in pregnancy: from implantation to parturition and beyond." Frontiers in Immunology 11 (2020): 1681. Distributed under Open Access license CC BY 4.0, without modification.

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