Overview Workflows Case study Related Products Featured Services Protocols Q&A Resources
Creative Biolabs uses cutting-edge technology and extensive scientific expertise to provide comprehensive functional test services for a wide range of complement components. Our high-quality, personalized complement C5a functional test is designed to meet the specific needs of each customer, offering precise, reliable data to support research and accelerate project success in immunology and therapeutic development.
Overview of C5a Functional Test
Complement C5a
C5a is a pro-inflammatory peptide generated during the activation of the complement system. It is generated through the cleavage of complement component C5 by the enzyme C5 convertase. C5a acts by binding to specific receptors (C5aR and C5L2) on immune cells, triggering chemotaxis, enhancing vascular permeability, and promoting the release of pro-inflammatory cytokines. It plays a crucial role in immune responses by attracting neutrophils and macrophages to sites of infection or injury, contributing to inflammation, tissue damage, and the defense against pathogens. C5a is also involved in autoimmune diseases and other inflammatory conditions.
Fig.1 The generation and function of C5a.1, 4
Complement C5a Functional Test
Method
The C5a functional test assesses the activity of the complement component C5a. Commonly used methods include C5a ELISA, which quantifies the concentration of C5a in serum or plasma, and chemiluminescence assays, which measure the activation of immune cells in response to C5a. The hemolysis assay involves testing C5a-induced red blood cell lysis. These methods help evaluate complement system activation and C5a's role in immune responses.
Analysis
Interpreting the C5a functional test involves analyzing C5a levels or activity in the sample. Elevated C5a concentrations may indicate excessive complement activation, suggesting inflammation, infection, or autoimmune disease. The test plays a crucial role in diagnosing conditions like sepsis, rheumatoid arthritis, and other immune disorders. It helps evaluate complement system functionality, guiding therapeutic interventions to manage inflammatory or immune-related conditions.
Workflow of C5a Functional Test
C5a ELISA
Hemolytic assay
Chemiluminescence assay
Case Study
Fig.2 Association between serum C5a levels and the presence of respiratory symptoms in COVID-19 Patients.2,4
Fig.3 The difference of serum C5a level between mild, moderate and severe Alzheimer’s disease patients.3,4
Related Products
Featured Services
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Total Complement Activity Test
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C1q-Binding Assays
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Creative Biolabs offers total complement activity test services for our clients. Total complement activity tests include CH50, AH50, and LP50.
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Creative Biolabs offers high-quality C1q-binding assays for the characterization of therapeutic mAbs.
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Learn more
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Learn more
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Protocols
Questions & Answer
A: C5a is a potent inflammatory mediator and an anaphylatoxin that triggers immune cell recruitment and activation, contributing to inflammation. In contrast, C5b initiates the formation of the membrane attack complex (MAC), leading to targeted cell lysis and pathogen destruction.
A: For complement C5a functional test, serum or plasma samples are typically required. A specific sample volume is typically required, and we will provide the details when the purchase order is placed. For more details, please reach out to our technical team. To prevent degradation, fresh samples are preferred, and appropriate storage is necessary to maintain stability before analysis.
A: Yes, we provide an all-inclusive, one-stop solution for complement C5a functional test. This includes sample collection, precise tests, data analysis, and expert interpretation, ensuring a seamless experience and reliable results for research applications.
A: The turnaround time for the complement C5a functional test is usually 1 to 2 weeks, depending on the specific test used and the laboratory's workflow. Our experts will discuss the experimental progress with you in a timely manner, and we will also provide expedited processing for your emergency test services.
Resources
Creative Biolabs provides one-stop functional test services for global customers, involving multiple methods of complement C5a functional test services and various supporting reagent products for rapid evaluation of complement activity. Contact us and our professional scientists will respond to you as soon as possible.
References
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Horiuchi, Takahiko, and Hiroshi Tsukamoto. "Complement-targeted therapy: development of C5-and C5a-targeted inhibition." Inflammation and regeneration 36 (2016): 1-5.
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Senent, Yaiza, et al. "Persistence of high levels of serum complement C5a in severe COVID-19 cases after hospital discharge." Frontiers in Immunology 12 (2021): 767376.
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Li, Zhilian, et al. "Correlation of serum complement factor 5a level with inflammatory response and cognitive function in patients with Alzheimer’s disease of different severity." BMC neurology 23.1 (2023): 319.
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Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
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