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Creative Biolabs offers customized, top-quality functional assays for complement C3 convertase, leveraging state-of-the-art technologies and extensive scientific expertise to assist clients in progressing complement system-targeted therapies.
Overview of Complement C3 Convertase
C3 convertases are protein assemblies generated either through the involvement of the activated C1 complex, C2, and C4 (C4b2a, classical activation pathway), or by C3 itself in conjunction with factors B and D (C3bBb, alternative pathway). These convertase enzymes play a crucial role in the complement cascade by cleaving C3 and C5, processes that are essential for nearly all complement effector functions. C3 convertases split C3 into C3a, a chemoattractant molecule, and C3b, which covalently attaches to target surfaces and initiates phagocytosis.
Fig.1 Complement convertases facilitate the cleavage of C3 and C5.1
Complement C3 Convertase Functional Assay Introduction
Method
The complement C3 Convertase functional assay is a laboratory approach devised to evaluate the activity and function of the C3 Convertase component within the complement system across a variety of samples. This assessment can be carried out using hemolytic tests and ELISA techniques. Additionally, we also offer SPR chips that are employed to analyze the sequential assembly and dissociation of convertases.
Analysis
In complement research, since all pathways converge at the convertase stage, one of the key functional assessments focuses on measuring convertase activities and examining the influence of specific components on these activities. Through the implementation of functional or activity assays for the C3 convertase, we can evaluate and analyze its function at a quantitative level.
Workflow of Complement C3 Convertase Functional Test
Applications
Creative Biolabs offers C3 convertase functional assays to support our esteemed clients in various applications, including: (1) elucidating the molecular biology of convertases, (2) characterizing the actions of known complement inhibitors, (3) examining the impact of deficiencies and mutations in complement proteins linked to diseases, (4) discovering novel and specific convertase inhibitors, and (5) facilitating the customization of anti-complement therapies to improve disease treatment.
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Questions & Answer
A: C3 convertase is an essential enzymatic assembly within the complement system, pivotal for initiating vital immune reactions through the cleavage of C3 and C5 proteins. This mechanism is crucial for immune defense processes such as phagocytosis and inflammation. At Creative Biolabs, we provide advanced assays to study its activity.
A: Absolutely, and we appreciate your question! Our assays are designed to support groundbreaking research into complement-targeted therapies, including discovering novel C3 convertase inhibitors. We’re dedicated to advancing your therapeutic developments with precision and expertise. Let’s collaborate.
A: We use advanced techniques like hemolytic tests, ELISA, and SPR chip analysis to evaluate C3 convertase activity and assembly. These methods provide detailed insights into the enzyme's function, helping researchers uncover critical immune mechanisms.
A: Yes, our assays are perfect for characterizing known complement inhibitors and discovering new ones, helping researchers tailor anti-complement therapies. Leveraging our specialized knowledge and state-of-the-art technology, we are poised to support you in progressing drug development.
A: The timeframe for obtaining results can vary based on the sample's complexity and the test's specifications. Generally, results are provided within ~1 month after the sample's receipt. If the initial results are inconclusive, we will contact you to discuss subsequent steps, which may involve re-testing the current sample or obtaining a new one.
Resources
Creative Biolabs is dedicated to providing effective and pragmatic complement-focused services, grounded in a dependable and standardized assay platform. We provide customized C3 convertase functional assays aimed at the swift evaluation of complement activity. We encourage you to contact us to explore any inquiries or interests you might have concerning the complement function assay.
Reference
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Zwarthoff, Seline A., et al. "Functional characterization of alternative and classical pathway C3/C5 convertase activity and inhibition using purified models." Frontiers in Immunology 9 (2018): 1691. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
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