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Creative Biolabs provides first-class and personalized complement C5b functional test services tailored to your research needs. Our advanced techniques and expertise ensure precise and reliable quantification of C5b activity, enabling you to gain valuable insights into immune system function and accelerate progress in your immune-related research projects.
Overview of C5b Functional Test
Complement C5b
Complement C5b is a key element of the complement system, playing a vital role in the innate immune defense. Upon activation, the C5 protein is cleaved into C5a and C5b. C5b triggers the assembly of the membrane attack complex (MAC) by sequentially binding to C6, C7, C8, and several C9 molecules. This complex forms a pore in the pathogen’s cell membrane, resulting in cell lysis and subsequent death. Beyond direct lytic activity, C5b aids in amplifying the inflammatory response and facilitates opsonization, thus enhancing pathogen clearance and maintaining immune system balance.
Fig.1 Schematic representation of MAC.Distributed under CC BY-SA 3.0, from Wiki, without modification.
Complement C5b Functional Test
Method
The Complement C5b functional test is designed to measure the activity of C5b in the immune system. The test is based on detecting the activation of complement components and the formation of C5b and MAC. The method involves isolating plasma or serum samples and using hemolytic assays, ELISA, or similar immunoassays to detect and measure C5b levels.
Analysis
The C5b functional test provides insight into complement system activation, specifically the initiation of the MAC. Elevated or altered C5b levels can indicate immune dysfunction or complement-related diseases. Accurate interpretation of the test helps assess the effectiveness of immune responses in both inflammatory conditions and infections. This test is crucial for understanding complement activation mechanisms and their role in autoimmune diseases, infectious diseases, and therapeutic development.
Workflow of C5b Functional Test
C5b ELISA
Hemolysis assay
Case Study
Fig.2 Increased levels of C5b-9 in COVID-19 patients.1
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Questions & Answer
A: The C5b functional test is critical for identifying conditions linked to complement system abnormalities. These include autoimmune diseases like Systemic Lupus Erythematosus (SLE), atypical hemolytic uremic syndrome (aHUS), paroxysmal nocturnal hemoglobinuria (PNH), and various infectious diseases where complement activation plays a key role. Additionally, it can be used to evaluate complement inhibition therapies and genetic complement deficiencies.
A: The C5b functional test is highly accurate when performed under controlled conditions, with proper sample handling and storage. Both the ELISA and hemolysis assay methods have been validated and yield consistent, trustworthy results. Calibration using standard controls ensures precise and reproducible measurements. However, factors like sample quality, individual patient conditions, or potential technical errors during the assay may impact accuracy.
A: The complement C5b functional test is essential in immunological research, particularly in studying the mechanisms of complement activation and regulation. By providing quantitative and qualitative insights into C5b activity, researchers can explore disease pathogenesis, evaluate new therapeutic targets, and assess the effects of various interventions on the complement pathway. This test is a valuable tool for studying immune responses across various disease models.
A: Yes, we offer customizable test solutions to address the specific requirements of your research projects. Whether you need batch tests, modified assay protocols, or detailed data analysis, our services can be tailored to align with your study's objectives. Our team is available to discuss any specific requirements and develop a bespoke test plan that fits your research goals and timelines.
A: Researchers receive comprehensive data reports, including raw and analyzed data in formats compatible with standard statistical software. These reports often include graphical representations of complement activity, comparative analyses, and detailed documentation of assay conditions. Our service aims to facilitate easy integration of test results into broader research databases and publications.
Resources
Based on our comprehensive complement function test platform, Creative Biolabs offers customized C5b functional test solutions, as well as robust results and timely data delivery. Contact us today to advance your complement research and accelerate the success of your projects.
Reference
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Detsika, Maria G., et al. "C3a and C5b-9 Differentially Predict COVID-19 Progression and Outcome." Life 12.9 (2022): 1335. Distributed under Open Access license CC BY 4.0. The image was modified by extracting and using only part of the original image.
For Research Use Only.
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