Overview Workflows Applications Related Products Featured Services Protocols Q&A Resources
Creative Biolabs delivers high-quality, tailored functional assays for complement C3a, harnessing advanced technologies and profound scientific expertise to aid clients in progressing complement-based therapeutic developments.
Overview of Complement C3a
Upon activation of the complement system through any pathway, enzyme complexes known as C3 convertases are formed, which cleave C3 into two fragments: C3a and C3b. The C3a fragment, composed of 75 amino acid residues, is released from complement C3 during system activation to stimulate the immune response. As the smaller fragment, C3a, akin to C5a which is subsequently generated, functions as a pro-inflammatory signaling molecule, known as an anaphylatoxin. Anaphylatoxins function as chemoattractants, drawing in and activating a range of inflammatory cells, such as mast cells and neutrophils.
Fig.1 Diagrammatic summary of the complement cascade: activation through the classical, lectin and alternative pathways converge at the assembly of C3 convertases.1
Complement C3a Functional Assay Introduction
Method
The complement C3a functional assay is a laboratory method crafted to assess the activity and functionality of the C3a component within the complement system across diverse samples, such as human serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants. This evaluation can be performed using hemolytic assays and solid-phase sandwich ELISA techniques.
Analysis
C3a serves as a pivotal chemotactic mediator in the immune system, with the C3a/C3aR pathway playing dual roles—anti-inflammatory and pro-inflammatory—across various cells and diseases. By performing functional or activity assays of C3a, its function can be rigorously evaluated and quantified. Statistical analyses, such as presenting values as the mean ± standard error or mean ± standard deviation, are employed to determine the significance of differences using unpaired and paired t-tests. Spearman's correlation test is utilized to compare plasma concentrations of C3a.
Workflow of Complement C3a Functional Test
Applications
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Detection of Diseases: The complement protein C3a has been implicated in various conditions, including retinal diseases and injuries. Its role is particularly pronounced in facilitating an anaphylactic response following laser-induced damage. Measuring C3a activity not only aids in diagnosing these conditions but also assists in monitoring the progression of the disease.
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As a Biomarker: C3a, a fragment released upon the activation of complement protein C3, binds to specific receptors on various cells, including phagocytes, to initiate multiple complement-mediated pro-inflammatory responses. This capability positions C3a as a potential biomarker for predicting diseases and investigating their pathogenesis.
Related Products
Featured Services
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Total Complement Activity Test
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Complement Multiplex Assay Services
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Creative Biolabs offers total complement activity test services for our clients. Total complement activity tests include CH50, AH50, and LP50.
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We offer specialized complement multiplex assay services for the characterization of complement proteins and the development of complement therapies, enabling simultaneous analysis of multiple complement proteins.
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Learn more
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Learn more
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Protocols
Questions & Answer
A: C3a is a small yet potent fragment within the complement system, serving a crucial role in immune responses as a pro-inflammatory signaling molecule. At Creative Biolabs, we deliver high-quality assays to assess its activity and functionality, helping clients in therapeutic research.
A: Yes, our C3a functional assays can provide essential insights into the role of C3a in retinal diseases and other conditions. These insights help researchers develop effective therapeutic strategies. With our advanced techniques, we’re here to support your research goals.
A: Our C3a functional assays are compatible with a diverse array of samples, such as human serum, tissue homogenates, and cell culture supernatants. Regardless of your research requirements, we offer tailored solutions to meet your needs.
A: Our assays use methods like hemolytic tests and solid-phase sandwich ELISA techniques to measure C3a activity. These methods allow us to evaluate immune responses in detail and provide precise, reliable data. Let us help advance your research.
Resources
Creative Biolabs is committed to offering efficient and practical complement-related services, grounded on a reliable and standardized assay platform. We deliver customized C3a functional assays for swift evaluation of complement activity. We welcome you to contact us to discuss any interests or questions you may have regarding the complement function assay.
Reference
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Gao, Shuang, Zhao Cui, and Ming-hui Zhao. "The complement C3a and C3a receptor pathway in kidney diseases." Frontiers in immunology 11 (2020): 1875. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
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