Human Complement C5a desArg ELISA Kit-CTK-828(Cat#: CTK-828)

Name: Human Complement C5a desArg ELISA Kit-CTK-828
SKU: CTK-828

This product is an ELISA kit for the determination of Human Complement C5a desArg in the samples of plasma, serum, and other biological samples. It is highly sensitive and available for the detection of Human Complement C5a desArg as low as 0.047 ng/mL.

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Specifications

Assay Type	Sandwich ELISA
Sensitivity	0.047 ng/mL (0.078-5.0 ng/mL)
Species Reactivity	Human
Target	C5a
Cross Reactivity	No significant cross-reactions have been observed between human complement C5 (~4.8%) and human complement C4 (~1.5%), but existing techniques may limit the detection of cross-reactions with untested analogues.
Applications	ELISA
Storage	Short-term storage at 4°C and long-term storage at -20°C.

Information

Introduction

Complement component 5a (C5a) is an inflammatory polypeptide produced from cleavage of complement component C5 by protease C5-convertase. Biologically, C5a is a potent anaphylatoxin and a chemotactic factor playing a critical role in inflammation. It has been demonstrated that C5a could increase vascular permeability and induce chemotaxis and the release of active mediators.
In human plasma or serum, C5a is rapidly "inactivated" or converted to C5a desArg by serum carboxypeptidase N through the removal of the C-terminal arginine (Arg) within minutes. Although C5a des-Arg is considered to be the inactivated form of C5a, C5a desArg retains significant biological activity including weak anaphylatoxic and chemotactic activities, inducing muscle contraction and vascular permeability increase, causing the release of histamine and lysosomal degranulation of immune cells.
As C5a desArg is the main active form of C5a in plasma and serum, the quantitation of C5a-desArg in samples is an indirect reflection or determination of the level of complement activation in the test samples.

Customer Review

It has never let us down

Excellent

The sensitivity and specificity are impressive, allowing us to detect even the smallest changes in C5a desArg levels. The protocol is straightforward and the results are consistent. We've used this kit for multiple projects.

This ELISA kit is one of the most reliable ones we've used in our lab

Excellent

The clear instructions and easy-to-follow protocol make it accessible even for less experienced staff. The reagents are of high quality, and the calibration curve is linear and reproducible. We have been able to generate accurate and reproducible data consistently.

Fantastic for measuring C5a desArg levels

Excellent

The assay is highly sensitive and specific, and the results are very reproducible. The kit comes with all the necessary components and the protocol is easy to follow. We've used it in several studies and have been very pleased with the performance.

A valuable addition to our lab

Excellent

We have used this kit for both human and animal samples and it has worked well for both. The assay is sensitive and provides consistent results. The instructions are easy to follow and the reagents are of high quality.

The assay is very sensitive, and the results are highly reproducible

Excellent

I've used many ELISA kits over the years. The kit is well-designed, with clear instructions and high-quality reagents. It has significantly improved our ability to measure C5a desArg levels in our studies.

For Research Use Only. Not for Diagnostic or Therapeutic Applications.