Klebsiella pneumonia-derived Exosome Research and Application

Klebsiella pneumonia-derived exosomes have been shown to be capable of loading and transporting drugs along with evoking the immune response, which facilitates the research and development of more effective therapeutic agent delivery vectors. Creative Biolabs has an extensive understanding of gram-negative bacterial-derived exosomes, with our scientific team continually dedicated to providing reliable research services on bacterial-derived exosomes.

Klebsiella pneumonia-derived Exosome Isolation

Culture Klebsiella pneumonia in sterilized LB medium overnight with shaking.
Centrifuge Klebsiella pneumonia cultures at low speed at 4°C to remove large parental bacteria.
Further filter the Klebsiella pneumonia supernatant and then concentrate with ultrafiltration membranes.
Concentrate the supernatant by ultracentrifugation and further purify the obtained Klebsiella pneumonia-derived exosomes by the sucrose density gradient method.
Remove endotoxin from Klebsiella pneumonia-derived exosomes, final filter sterilization, and store at -80℃.

Studies of Klebsiella pneumonia-derived Exosome

Research	Conclusion
Characterization of Klebsiella pneumonia-derived exosomes before and after packaging of drugs.	Observation by transmission electron microscopy and analysis of particle size by DLS revealed that there was no significant change in the homogeneous characterization of Klebsiella pneumonia-derived exosomes before and after successful encapsulation of the drug, whereas there was an appropriate increase in particle size.
Klebsiella pneumonia-derived exosomes carried drugs to tumor cells.	In non-small cell lung cancer cell lines and subcutaneously injected hormonal mice, it was demonstrated by confocal microscopy that tumor cells efficiently uptook the drug-loaded Klebsiella pneumonia-derived exosomes. Moreover, Klebsiella pneumonia-derived exosomes as carriers improved drug uptake and transport by tumor cells compared to free drugs.
Klebsiella pneumonia-derived exosomes delivered drug antitumor efficacy.	Klebsiella pneumonia-derived exosomes encapsulating chemotherapeutic drugs delivered more cytotoxicity in tumor cells and induced more dose-dependent apoptosis compared to free drugs. Also, intraperitoneal injection of Klebsiella pneumonia-derived exosomes encapsulating chemotherapeutic drugs induced massive apoptosis and extensive tumor necrosis in tumor tissues in the disease mouse model.
Klebsiella pneumonia-derived exosomes transmitted auto-immunogenicity to attack tumors.	Treatment of empty Klebsiella pneumonia-derived exosomes also elicited high levels of F4/80 protein in mouse tumor tissues, suggesting macrophage recruitment in the tumors. Further results of serum immune cytokine analysis confirmed that empty Klebsiella pneumonia-derived exosomes themselves triggered the host's immune response, synergistically exerting anti-tumor effects.
Klebsiella pneumonia-derived exosomes improved the pharmacokinetic profile of chemotherapeutic agents.	The results of pharmacokinetic parameter analysis showed that chemotherapeutic drugs encapsulated in Klebsiella pneumonia-derived exosomes showed slower decay rate, lower clearance, and lower release concentration. This suggests that Klebsiella pneumonia-derived exosomes favor in vivo stability and improved pharmacokinetics of chemotherapeutic drugs.

Fig. 1 Klebsiella pneumonia-derived exosomes loaded with chemotherapeutic drugs exhibited antitumor effects.¹

With the ability to deliver drugs to the focal area while carrying natural immunogenicity that induces macrophage recruitment, Klebsiella pneumonia-derived exosomes have the potential to act as carriers of encapsulated drugs for chemo-immunization to inhibit tumors. Creative Biolabs can provide customized research services for the production and analysis of Klebsiella pneumonia-derived exosomes. Please contact us to learn more.

Reference

Kuerban, Kudelaidi, et al. "Doxorubicin-loaded bacterial outer-membrane vesicles exert enhanced anti-tumor efficacy in non-small-cell lung cancer." Acta Pharmaceutica Sinica B 10.8 (2020): 1534-1548.

Plant Exosome Isolation