The smaller, more tractable and widely available alpaca is an excellent source of heavy-chain antibody (V_HH). As a long-term expert in the field of immunology, Creative Biolabs offers the unparalleled mass sequencing service to analyze the B-cell receptor (BCR) repertoires. Based on our advanced Magic™ BCR repertoire analysis platform, our scientists are pleased to introduce the service of BCR repertoire profiling in alpaca to all our global customers.

The Potential Application of Alpaca Antibodies

Fig.1 NGS-based BCR repertoire profiling followed by pairwise BCR sharing analysis.¹

Alpaca V_HHs are widely known to have highly favorable properties as antigen binding agents for research and commercial applications, but the poor accessibility, large size, expense and difficult handling that characterizes the use of camels and llamas has significantly limited their general use by scientists. Alpacas are more widely available, less expensive to maintain, smaller and more tractable than camels and llamas. The use as a source of immune B cells for V_HH library construction has expanded the accessibility of V_HHs to both in lab research and in the biopharmaceutical field. Studies on fetal alpaca revealed that the bone marrow serves as a site for heavy-chain and conventional B-cell lymphopoiesis; however, the spleen appeared to be the most active and likely functions to support early development, expansion, and possibly, class switching of B lymphocytes. In adult alpacas, the distribution of heavy-chain Ab-producing B cells among and within secondary lymphoid tissues is similar to that of conventional B cells. In addition to conventional immunoglobulins, alpacas produce antibodies that do not incorporate light chains into their structures. This class of IgG is able to bind to antigens despite the absence of a heavy chain C_H1 domain and the inability to combine with light chains. It is believed that V_HH arose by the loss of a splice consensus signal in the C_H1 exon of an ancestral alpaca. These V_HH antibodies have incited great interest in the biomedical community, as they have considerable potential for biotechnological and therapeutic application.

Alpaca BCR Repertoire Profiling in Creative Biolabs

Equipped with world-leading technology platforms and professional scientific staff in the field of immunology, Creative Biolabs has rich experience in BCR repertoire profiling in alpaca. Our seasoned scientist can provide a comprehensive analysis of BCR repertoire in alpaca. Specific capture primers to CDRs in alpaca are used for amplification with high fidelity. PBMC or RNA from it can be good starting materials.

Key Advantages of Our Alpaca BCR Repertoire Profiling Service

• Small amount of sample
• High-fidelity amplification of CDRs in alpaca
• Both the heavy chain and the light chain CDR 3 regions can be 100% covered
• Advanced Magic™ sequencing platform

In terms of the extensive experience in BCR repertoire analysis in alpaca, Creative Biolabs is proud to provide a comprehensive analysis of BCR repertoire in alpaca with the best quality and most competitive price.

Please contact us for more information and a detailed quote.

Diverse Species of Our Unbiased BCR Repertoire Profiling

• Feline Immunoglobulin Repertoire Profiling
• Zebrafish Antibody Repertoire Analysis
• Canine BCR Repertoire Profiling
• Rabbit BCR Repertoire Profiling
• Swine BCR Repertoire Profiling

Applications of Our Unbiased BCR Repertoire Profiling

• B Cell Clones Identification
• Novel Alleles Identification
• SHM Patterns Analysis

FAQ

1. What is alpaca BCR repertoire profiling

   Alpaca BCR (B cell receptor) repertoire profiling is a method used to study the diversity and composition of B cell receptors in alpacas. This technique typically involves high-throughput sequencing to identify various BCRs, allowing researchers to understand the immune response mechanisms in alpacas and their potential applications in antibody discovery and immunology research.

2. Why are alpacas used in BCR repertoire profiling

   Alpacas are valuable in BCR repertoire profiling because they produce unique antibodies, including single-domain antibodies. These VHHs are smaller and more stable than conventional antibodies, making them useful in research and therapeutic applications. Alpaca BCR profiling helps explore these immune properties, which could lead to the development of new diagnostics and treatments.

3. How does BCR repertoire profiling in alpacas contribute to antibody discovery

   Alpacas produce a special class of antibodies known as VHH, which are highly sought after for research and therapeutic use. BCR repertoire profiling allows scientists to map the diverse antibody responses in alpacas, providing insights into the generation of specific VHHs that can be used to develop treatments for diseases like cancer, infectious diseases, and more.

4. What technologies are used in alpaca BCR repertoire profiling

   Next-generation sequencing (NGS) is commonly used to perform alpaca BCR repertoire profiling. This technology enables comprehensive analysis of the diverse B cell populations in alpacas, capturing detailed information about the variety and clonality of B cell receptors. Advanced bioinformatics tools are then employed to analyze the sequencing data, helping researchers interpret immune responses.

5. What makes alpaca sdAbs distinct from traditional antibodies, and how is this linked to BCR profiling

   Alpaca sdAbs are distinct due to their small size, high stability, and ability to bind to unique epitopes that traditional antibodies cannot reach. BCR repertoire profiling helps researchers identify and analyze these sdAbs by mapping the specific B cell receptors that produce them, offering opportunities for designing novel therapeutic and diagnostic tools.

6. What role does BCR repertoire profiling play in the study of alpaca immune responses to vaccines

   BCR repertoire profiling allows researchers to analyze the immune response of alpacas to various vaccines. By examining the diversity and evolution of B cell receptors before and after vaccination, scientists can identify effective immune responses and VHHs, contributing to the design of more efficient vaccines or improving existing ones for both veterinary and human applications.

Resources

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