Creative Biolabs offers the unparalleled mass sequencing service to analyze the antibody repertoires of zebrafish. According to our Magic™ platform based on next-generation sequencing (NGS) technique, our scientists are able to perform a comprehensive analysis of the heavy-chain antibody repertoire of zebrafish.

The Background of Zebrafish Immune System

The antibody repertoire generated by the immune system can be directed to create antibodies with broad chemical diversity and high selectivity. There already has been a good understanding of the potentially available diversity and the mechanistic aspects of how this diversity is generated. Antibodies are composed of heavy chains and light chains, each containing a highly diversified antigen binding domain. The V, D and J gene segments of the antibody heavy chain variable genes go through a series of recombination events to generate a new heavy chain gene. Since zebrafish shares great homology to the human genome, it is an ideal model system for studying the adaptive immune system. Like human, zebrafish has a combinatorial rearrangement of V, D and J gene segments to create antibodies. It also has junctional diversity during recombination and somatic hypermutation of antibodies to improve specificity, and the organization of its immunoglobulin (Ig) gene loci approximates that of human. Each individual's immune system is an independent experiment in evolution by natural selection. It is still unclear what fraction of the potential repertoire is expressed in an individual zebrafish at any point in time.

Fig.1 B-cell subtype analysis based on single-cell gene expression.¹

Zebrafish Antibody Repertoire Analysis in Creative Biolabs

Creative Biolabs has established the high-throughput Magic™ platform for large-scale sequencing services. Our scientists have developed an approach to defining the antibody repertoire of zebrafish by analyzing complementarity determining region 3 (CDR3) of the heavy chain. mRNA is prepared from the whole fish, and then cDNA is synthesized by using primers designed to capture the entire variable region. The antibody repertoire library will be sequenced on our high-throughput Magic™ platform. Our scientists focus our analysis on CDR3 sequences. This approach provides insight into the breadth of the expressed antibody repertoire and immunological diversity at the level of an individual zebrafish.

Key Advantages of Our Zebrafish Antibody Repertoire Analysis Services

• Single base detection
• None bias PCR, 5' RACE
• Coverage of the entire variable region
• Rapid turnaround time
• High-throughput Magic™ platform

As a long-term expert in the field of immunology, Creative Biolabs has owned lots of scientists who are proficient in analyzing the zebrafish antibody repertoire. We are confident in providing clients with the best service at the most competitive cost.

Please contact us for more information and a detailed quote.

Diverse Species of Our Unbiased BCR Repertoire Profiling

• Feline Immunoglobulin Repertoire Profiling
• Alpaca BCR Repertoire Profiling
• Canine BCR Repertoire Profiling
• Rabbit BCR Repertoire Profiling
• Swine BCR Repertoire Profiling

Applications of Our Unbiased BCR Repertoire Profiling

• B Cell Clones Identification
• Novel Alleles Identification
• SHM Patterns Analysis

FAQ

1. What is zebrafish BCR repertoire profiling

   Zebrafish BCR (B cell receptor) repertoire profiling is a method to analyze the diversity and composition of B cell receptors in zebrafish. This involves high-throughput sequencing techniques to study the variations in the BCR sequences, which can provide insight into how the immune system responds to infections, cancers, and autoimmune diseases using zebrafish as a model organism.

2. Why is zebrafish a suitable model for studying human diseases through BCR repertoire profiling

   Zebrafish have immune systems that are remarkably similar to those of humans, particularly in adaptive immunity. Their transparent embryos and rapid development allow researchers to observe disease progression in real-time. BCR repertoire profiling in zebrafish is particularly valuable for studying immune responses to cancers, infectious diseases, and immunodeficiencies, offering a practical model for investigating human immune disorders.

3. How does BCR repertoire profiling in zebrafish help in cancer research

   Zebrafish BCR repertoire profiling allows researchers to investigate how the immune system responds to tumor growth. Zebrafish models have been used to study leukemia, melanoma, and other cancers. By analyzing the BCR diversity, researchers can understand how B cells are activated during tumor development and how the immune system could potentially be harnessed for cancer immunotherapy.

4. What technologies are used for zebrafish BCR repertoire profiling

   High-throughput sequencing technologies such as next-generation sequencing (NGS) are used to analyze the BCR repertoire in zebrafish. These methods allow for detailed examination of the diversity, clonal expansion, and evolution of B cell populations in response to disease. Data analysis tools specific to immune profiling are then used to interpret the results, revealing patterns of immune activation or suppression.

5. How does the transparency of zebrafish benefit BCR repertoire profiling in disease research

   The transparency of zebrafish embryos allows for direct observation of immune responses, making it possible to visualize interactions between B cells and pathogens or cancer cells in real-time. This feature, coupled with BCR repertoire profiling, enables a more comprehensive understanding of how the immune system combats diseases, offering insights into the development of effective immunotherapies.

6. What role does zebrafish BCR repertoire profiling play in personalized medicine

   BCR repertoire profiling in zebrafish contributes to the development of personalized medicine by allowing researchers to model patient-specific immune responses. Using patient-derived cells or genetic material, zebrafish can be used to study individualized immune reactions to specific diseases, helping to tailor treatments such as immunotherapies or targeted drug therapies based on a patient's unique immune profile.

7. What are the limitations of zebrafish BCR repertoire profiling in disease research

   While zebrafish provide a valuable model for immune research, there are limitations. Zebrafish immune systems, though similar, are not identical to those of humans, which may affect the translation of findings. Additionally, certain disease models may not fully replicate human pathology. Despite these limitations, zebrafish BCR repertoire profiling remains a powerful tool for studying immune responses and developing treatments for various diseases.

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