Porcine (Pig) BCR Repertoire Profiling Service

Background Swine BCR Repertoire Profiling Advantage Resources

With years of research and development experience in the field of immunology, Creative Biolabs has owned a group of scientists who have deep understanding of the adaptive immune system. Through our advanced one-stop BCR repertoires analysis service, our seasoned scientists can provide the best service of BCR repertoire profiling in swine. We are proud to share our expertise and experience with our global clients to facilitate their related immune repertoire researches.

The Properties of Antibody Repertoire in Swine

Studies have shown that newborn swine utilize primarily four V_H genes and two DH segments to generate their preimmune natural antibody repertoire. In the fetus, VDJ rearrangements are ﬁrst seen in yolk sac at 20 days of gestation. However, signal joint circles are difﬁcult to recover currently, probably because of the slow rate of B cell lymphogenesis at this early time and the rapid rate of their degradation. Since swine have only a single J_H, 30 combinatorial diversity is limited to essentially eight combinations. VDJ rearrangement was first observed on day 30 in fetal swine and all major isotypes of immunoglobulins are transcribed in utero. Swine has been used to better understand B-cell lymphogenesis in biomedical research, especially where antibody responses are involved. The piglet model has provided useful information on the B cell system for studies on antibody repertoire development. The process is too complicated and the results are always inconsistent with each other.

Fig.1 Workflow of single B cell sequencing. Fig.1 Single B cell transcriptomics for exploring pig B cell repertoire.¹

Swine BCR Repertoire Profiling in Creative Biolabs

High-throughput NGS has been widely used for the study of antibody repertoires. The diversity of the sequences obtained from these studies is immense, and thus efficient bioinformatics techniques to analyze and interpret the data are also required. Creative Biolabs has established the high-throughput Magic™ platform for antibody repertoires analysis in swine. Our scientists have also designed advanced algorithm for analyzing Ig gene sequences in swine obtained by sequencing. With high throughput sequencing and superior bioinformatics analysis tools, our Magic™ platform is powerful in analyzing a large number of DNA sequences present in the expressed antibody repertoire.

Key Advantages of Our Swine BCR Repertoire Profiling Service

Comprehensive BCR repertoire analysis from RNA of PBMC, bone marrow or spleen from swine
Specific primers to swine
High fidelity amplification method
Fast turnover time
Highly professional Ph.D. level scientists
Advanced Magic™ platform

Equipped with world-leading technology platforms and professional scientific staffs, Creative Biolabs has rich experience in BCR repertoire profiling in swine. We are pleased to share our cutting-edge technology and extensive expertise in BCR repertoire profiling in swine to facilitate our clients' research and project development.

Please contact us for more information and a detailed quote.

Diverse Species of Our Unbiased BCR Repertoire Profiling

Applications of Our Unbiased BCR Repertoire Profiling

Reference

Bram, Stanley, et al. "Parallel single B cell transcriptomics to elucidate pig B cell repertoire." Scientific reports 14.1 (2024): 15997. Distributed under Open Access license CC BY 4.0. The image was modified by revising the title.