Targeting Cytosol

Targeted delivery of substances into cells has long been hampered by the plasma membrane, which is an efficient barrier to exclude most molecules that are not actively imported by living cells. The discovery of CPP (cell penetrating peptide) opened up new possibilities for the translocation of larger molecules through the plasma membrane. Armed with innovative and diversified module delivery systems, a good approach can be accomplished by combining the module targeting cell surface with CPP targeting cytosol together. Creative Biolabs provides a wide range of cytosol-targeted delivery services for the customers all over the world.

The strategy of Cytosolic Targeting

Delivery of macromolecular drugs or smaller bioactive molecules is an important field in medical research. CPPs are unique tools to gain access to the cell interior and deliver a bioactive cargo into the cytosol. CPPs may have implications in biomedical sciences by improving the delivery of a wide variety of drugs through the membrane barrier. CPPs initially bind to negatively charged head groups of lipids or proteins in the plasma membrane followed by their internalization. Advances in recent years imply uptake mechanisms that are predominantly endocytotic. Normally, any alteration introduced in the peptide sequence will have a direct effect on the intracellular localization profiles.

Fig.1 The CPP-cargo complex enters the cytosol through different routes.¹

A novel, cationic cysteine-rich CPP, cytosol localizing peptide-1 (CyLoP-1), has been developed and it exhibits distinguished diffused cytosolic distribution along with endosomal uptake at low micromolar concentrations. The efficient intracellular delivery and the distribution into the entire cytosol make it an appropriate candidate for cytosolic delivery of small sizes cargoes.

What Can We Do for You?

Equipped with our well-established module delivery systems, we can provide either individual targeting modules or different kinds of module-payload/carrier conjugates targeting cytosol. A wide spectrum of corresponding products are also available. With years of unremitting efforts, Creative Biolabs has accumulated CPPs with different subcellular organelles targeting function. Moreover, we have modified and optimized the original sequence, thus forming our own unique CPP database. Flexible use of different delivery systems and CPPs to form different combinations can be a promising approach for selected delivery services targeting cytosol. We are more than happy to provide high-quality services and products to meet our client's specific requirements. If you are interested in our service, please do not hesitate to contact us for more details.

Reference

1. Hasannejad-Asl, Behnam, et al. "Cell penetrating peptide: A potent delivery system in vaccine development." Frontiers in Pharmacology 13 (2022): 4834.

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