This product is a PE conjugated anti-Human Complement C1Q Monoclonal antibody (TJL-03) generated from the Mouse. The antibody can be used for IF, IHC, FCM.
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| Clonality | Monoclonal |
| Clone | TJL-03 |
| Host Animal | Mouse |
| Isotype | IgG1 Kappa |
| Immunogen | Human Complement C1q |
| Species Reactivity | Human |
| Applications | IF; IHC; FCM |
| Application Notes | IF: 1:50-1:500 |
| Specificity | This antibody reacts with Human Complement C1q. |
| Purity | ≥95% as determined by SDS-PAGE |
| Conjugation | PE |
| Format | Liquid |
| Size | 200 µg |
| Storage | Store in dark at 4°C. Do Not Freeze. |
| Type | Primary Antibody |
| Target Name | C1Q |
| Alternative Names | Complement C1q; C1q complex |
| Introduction | The complement component 1q (C1q) is a protein complex with 18 peptide chains in 3 subunits, which are C1qA, C1qB, and C1qC. Meanwhile, the C1q, together with C1r and C1s, is a subunit of the C1 enzyme complex serving as an activator in the complement system. The C1q complex was activated once it binds to antigen-antibody complexes in an immune response. Additionally, the C1q complex tends to be attached to the immunoglobulin complement fixation sites, which are located in the CH2 domain of IgG, and the CH4 domain of IgM. As an important member of the complement system, C1q deficiency is closely associated with Lupus Erythematosus and Glomerulonephritis. |
I highly recommend this antibody for anyone working on complement system researchExcellentThe results were outstanding, with clear and specific staining of C1q. The antibody worked well at a dilution of 1:200 and provided bright fluorescence signals. Additionally, the background staining was minimal, which is always a plus.
Overall, it's a reliable and robust antibody for FCM applicationsExcellentWe used it for flow cytometry to study C1q expression on various cell types. The antibody's PE conjugation provided excellent fluorescence intensity, making it easy to distinguish positive cells from the background. It also showed high specificity, with no cross-reactivity with other complement components.
This product is definitely a valuable addition to our IHC toolkitExcellentWe employed the antibody in immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections. The antibody produced strong, specific staining of C1q with very low background. The PE label was bright and stable throughout the staining process.
Using the TJL-03 antibody for flow cytometry has been a pleasureExcellentThe PE conjugation ensures that the fluorescence signal is robust and easy to detect. The antibody binds specifically to C1q without any significant off-target effects. It's also easy to titrate to the optimal concentration, making it very user-friendly. I would definitely purchase this antibody again.
We are very satisfied with this productExcellentIn our recent study, we needed a reliable antibody to detect C1q in human serum samples by immunofluorescence. The antibody exceeded our expectations. The antibody provided strong, specific staining and was easy to work with. The bright PE signal made it straightforward to quantify C1q levels accurately.