VBS++ (with Ca and Mg) Buffer(Cat#: CTR-004)
VBS++ is like the traditional buffer used in complement assays (GVB++) but it lacks the carrier protein gelatin often used in these assays. GVB may be replaced with VBS if gelatin is undesirable or may interfere with the assays. The concentrations of metal ions are the traditional concentrations and these give maximal complement activation.
Summary
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Specifications
| Application |
VBS++ may be used when a buffer free of carrier proteins is needed for diluting proteins or as the buffer for complement assays. VBS++ may also be used to prepare GVBo, GVB++, and GVBE or it may be used to prepare similar complement buffers that include different carrier proteins in place of gelatin in situations where gelatin interferes with assays. BSA (0.1%) or another carrier protein may be used for this. |
| Composition |
5 mM Veronal, 145 mM NaCl, 0.025 % NaN3, pH 7.3. Containing 0.15 mM calcium chloride and 0.5 mM magnesium chloride. |
| Form |
Liquid |
| Storage |
Store at 4°C and avoid freezing. |
Customer Review
The stability of VBS++ buffer is unmatched
Excellent
VBS++ buffer has been incredibly reliable for our complement assays. The Ca and Mg composition ensures stable conditions, crucial for reproducibility in our experiments.
It's optimized for complement assays
Excellent
We've used VBS++ for years in our complement fixation assays. It remains stable over extended periods, which is critical for longitudinal studies.
It's easy to use, maintains optimal conditions for complement activation
Excellent
I've found VBS++ to be indispensable for studying complement pathways. The inclusion of Ca and Mg ions supports natural physiological conditions, enhancing assay accuracy.
Offer superior results compared to generic buffers
Excellent
As a lab manager, I appreciate buffers that simplify our protocols. VBS++ with Ca and Mg is straightforward to prepare and ensures precise control over our experimental variables.
For Research Use Only. Not for Diagnostic or Therapeutic Applications.
