Characterization of cofactor activity involves the protein interacting with C3b or C4b, molecules that can be cleaved by factor I (FI) to produce the inactive forms iC3b, C3c, C3dg, C4d and C4c. Therefore, incubating the cofactor and FI with the substrates C3b or C4b and assessing protein cleavage by SDS-PAGE is a simple and common strategy to demonstrate cofactor activity. This method plays a crucial role in the characterization of fluid-phase cofactors, such as factor H (FH) and C4bp, or cell-associated cofactors, such as membrane cofactor protein (MCP) and complement receptor 1 (CR1). Creative Biolabs has long been committed to complement research. Equipped with world-leading technology platforms and professional scientific researchers, our expert team can assist you in designing the best customized research plan to meet the requirements of your complement project.
Cofactor Activity Assay Protocol
Here, we are willing to share a simple protocol for cofactor activity assay.
Fig.1 Flow chart cofactor activity assay.
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