This product is an unconjugated anti-Human Complement C5b-9 Monoclonal antibody (CTJS-605) generated from the Mouse. The antibody can be used for ELISA; IF; IHC; WB.
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
| Clonality | Monoclonal |
| Clone | CTJS-605 |
| Host Animal | Mouse |
| Isotype | IgG2aκ |
| Immunogen | Purified human complement component C5b-9 |
| Species Reactivity | Human; Baboon; Horse; Porcine |
| Applications | ELISA; IF; IHC; WB |
| Application Notes | ELISA: 1:100-1:2000 IHC: 1:10-1:500 The optimal working dilutions should be determined by the end user. |
| Specificity | This antibody reacts with Human, Baboon, Horse, Porcine both membrane-bound MAC and fluid phase SC5b-9 complexes. |
| Purity | ≥95% as determined by SDS-PAGE |
| Format | Liquid |
| Size | 25; 100 µg |
| Storage | 4°C, store in dark |
| Type | Primary Antibody |
| Target Name | C5b-9 complex |
| Alternative Names | Membrane attack complex; MAC; Terminal complement complex; TCC; scC5b-9 |
| Introduction | C5b-9 complex is composed of the complement components C5b, C6, C7, C8 and several C9 molecules. It is a complex of proteins typically formed on the surface of pathogen cell membranes as a result of the activation of the host's complement system, and as such is an effector of the immune system. Assembly of the MAC leads to pores that disrupt the cell membrane of target cells, leading to cell lysis and death. |
Excellent for immunofluorescence applicationsExcellentWe used it to stain tissue sections and were very pleased with the clear and bright signals. The antibody binds specifically to its target without any non-specific background, which is crucial for our research on complement-mediated tissue damage.
It worked exceptionally wellExcellentIn our recent study on chronic inflammatory diseases, the antibody has proven to be a reliable tool. We used it for Western blotting and found it to be very effective. The bands were sharp and specific, allowing us to accurately quantify the C5b-9 complex levels in our samples.
This antibody has been an invaluable resource for our ELISA assaysExcellentWe've used it to quantify C5b-9 levels in patient serum samples, and the results have been highly consistent and reliable. The antibody's sensitivity allows for accurate detection even at low concentrations.
It's a high-quality product that we will continue to useExcellentIn our lab, we've used this antibody for immunofluorescence and were extremely satisfied with its performance. The staining was clear and specific, which helped us visualize the C5b-9 complex in various cell types.
I am very satisfied with its performanceExcellentThis antibody has proven to be very effective in Western blotting. The bands were clear and specific, with minimal background noise. It has helped us accurately quantify the C5b-9 complex in various experimental conditions.